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目的探讨7-硝基吲唑(7-NI)对创伤性颅脑损伤(TBI)大鼠血清神经元特异性烯醇化酶(NSE)和星形胶质源性蛋白(S100β)水平的影响。方法健康成年SD大鼠160只随机均分为生理盐水处理组和7-NI处理组;每组又均分为假手术和TBI后6、12和24h四小组。建立大鼠可控性皮质损伤模型。检测指标:血清NSE和S100β蛋白浓度,脑组织一氧化氮(NO)含量和神经元型一氧化氮合酶(nNOS)活性,脑组织含水量,血-脑脊液屏障(BBB)通透性,脑组织丙二醛(MDA)和总超氧化物歧化酶(T-SOD)含量。HE染色评价脑损伤严重程度。结果随TBI后时间推移,两组大鼠TBI后血清NSE和S100β蛋白浓度、脑组织NO含量和nNOS活性、脑组织含水量、BBB通透性和MDA含量均较假手术组增高(P<0.05),脑组织T-SOD含量下降(P<0.05),脑损伤逐步加重。在TBI后6h和12h,7-NI处理组血清NSE和S100β蛋白浓度、脑组织NO含量、脑组织nNOS、活性脑组织含水量、BBB通透性和MDA含量均低于生理盐水处理组(P<0.05);在伤后6h和12h,7-NI处理组脑组织T-SOD含量高于生理盐水处理组(P<0.05);在TBI后12h内,7-NI处理组脑损伤程度低于生理盐水处理组(P<0.05)。结论 TBI后早期应用7-NI可以减轻脑组织水肿,降低BBB通透性,减少脑组织氧化应激。血清NSE和S100β蛋白浓度可以反映7-NI的治疗效果。
Objective To investigate the effect of 7-nitroindazole (7-NI) on the levels of serum neuron-specific enolase (NSE) and astrocyte-derived protein (S100β) in traumatic brain injury (TBI) rats. Methods 160 healthy adult SD rats were randomly divided into normal saline group and 7-NI treated group. Each group was divided into sham operation and 6, 12 and 24 hours after TBI. Establishment of rat controllable cortical injury model. Serum NSE and S100β protein levels, brain tissue nitric oxide (NO) content and neuronal nitric oxide synthase (nNOS) activity, brain water content, blood-brain barrier (BBB) permeability, brain Tissue malondialdehyde (MDA) and total superoxide dismutase (T-SOD) content. HE staining was used to assess the severity of brain injury. Results After TBI, the levels of serum NSE and S100β, NO and nNOS activity, brain water content, BBB permeability and MDA content in TBI group were higher than those in sham operation group after TBI (P <0.05) ), The content of T-SOD in brain tissue decreased (P <0.05), and the brain injury gradually increased. At 6h and 12h after TBI, the levels of serum NSE and S100β, NO content in brain tissue, nNOS, brain water content, BBB permeability and MDA content in 7-NI treatment group were lower than those in saline control group (P <0.05). The content of T-SOD in brain tissue of 7-NI treated group was higher than that of saline treated group (P <0.05) at 6h and 12h after injury. The extent of brain injury was lower in 7-NI treated group Saline treatment group (P <0.05). Conclusion The early application of 7-NI after TBI can reduce brain edema, reduce BBB permeability and reduce oxidative stress in brain tissue. Serum NSE and S100β protein concentrations reflect the therapeutic effect of 7-NI.