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目的 评估世诺二代肌酸激酶同工酶-MB(CK-MB)试剂(CK-S、CKMB-S)抑制CK-MB活性假性升高的性能.方法 收集申能德赛CK-MB试剂(CK-D、CKMB-D)筛查异常血清90 例,使用CK-S、CKMB-S重测,为实验组;CK-D、CKMB-D复检结果为对照组.采用日立7600全自动生化分析仪检测,均为免疫抑制法.结果 CKMB-S/CK-S×100%多分布在0%～30%,CKMB-D/CK-D多分布在＞30%的区域.CKMB-D/CK-D、CKMB-S/CK-S倒置率分别为25.6%、4.4%, χ2＝15.730,P＜0.01;比例异常率分别为56.7%、14.4%,χ2＝35.011,P＜0.01;大于参考区间上限率分别为17.8%、8.9%,χ2＝3.077,P＞0.05.结论 添加了抗线粒体CK抗体的CK-S、CKMB-S试剂,使CK-MB检测结果更接近于真值.“,”Objective The second generation creatine kinase isoenzyme-MB (CK-MB) reagent (CK-S, CKMB-S) was evaluated for its ability to inhibit the pseudo-increased activity of CK-MB activity. Methods Ninety patients with abnormalities detected by Shenneng Desai CK-MB reagent (CK-D, CKMB-D) were collected and retested by CK-S, CKMB-S for the experimental group; CK-D, CKMB-D result of the retest was for the control group. 7600 automatic biochemical analyzer was used, all with immunosuppression. Results CKMB-S/CK-S ×100% distribution was in 0%～30%, CKMB-D/CK-D distribution in ＞30%. The conversion rates of CKMB-D/CK-D and CKMB-S/CK-S were 25.6% and 4.4%, respectively, χ2＝15.730, P＜0.01; the abnormal rates were 56.7% and 14.4%, respectively, χ2＝35.011, P＜0.01; the upper limit ratio is greater than the reference interval of 17.8%, 8.9%, χ2＝3.077, P＞0.05. Conclusion The addition of CK-S, CKMB-S reagents of MtCK makes the CK-MB detection result closer to the true value.