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目的检测URSA患者经主动免疫治疗前后外周血中sHLA-G的水平表达,为主动免疫治疗对URSA患者疗效评价提供一定的理论及方法学依据。方法将研究对象分为四组,URSA组、URSA主动免疫治疗组、正常早孕组及正常未孕组。用ELISA法定量检测样本中sHLA-G的表达情况,RT-PCR半定量检测外周血中sHLA-G的表达情况。结果(1)正常早孕组血清中sHLA-G的表达水平显著高于原因不明性流产组以及正常未孕组(P<0.05);URSA患者主动免疫治疗组sHLA-G的表达水平高于原因不明性流产组以及正常未孕组(P<0.05),与正常早孕组相比无统计学意义(P>0.05);(2)RT-PCR半定量检测显示URSA组及正常未孕组sHLA-G表达均低于正常早孕组及治疗组(P<0.05)。结论淋巴细胞主动免疫治疗可以提高外周血中sHLA-G的表达,主动免疫治疗能够有效治疗URSA妊娠后的流产患者。
Objective To detect the expression of sHLA-G in peripheral blood of patients with URSA before and after active immunotherapy, and to provide some theoretical and methodological evidences for the evaluation of the efficacy of active immunotherapy in patients with URSA. Methods The subjects were divided into four groups, URSA group, URSA active immunotherapy group, normal early pregnancy group and normal non-pregnant group. The expression of sHLA-G was quantitatively detected by ELISA. The expression of sHLA-G in peripheral blood was detected by RT-PCR. Results (1) The serum levels of sHLA-G in normal pregnancy group were significantly higher than those in unexplained abortion group and normal non-pregnant group (P <0.05). The expression level of sHLA-G in active immunization group was higher than that in unexplained group (P> 0.05). (2) RT-PCR semi-quantitative analysis showed that sHLA-G in URSA group and normal non-pregnant group The expression was lower than that of the normal pregnancy group and the treatment group (P <0.05). Conclusion Active immunotherapy with lymphocytes can increase the expression of sHLA-G in peripheral blood. Active immunotherapy can effectively treat the abortion patients after URSA pregnancy.