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目的探讨姜黄素对人胰腺癌PANC-1细胞甲基化转移酶(DNMTs)基因表达的影响及其作用机制。方法将体外培养的PANC-1细胞分为对照组、吉西他滨组、姜黄素组和联合组。各组分别干预48 h后,采用CCK8法检测人胰腺癌PANC-1细胞的增殖情况,采用RT-PCR法检测DNMTs mRNA表达情况,采用Western blotting法检测DNMT1和Caspase-3蛋白表达水平。结果 1联合组PANC-l细胞增殖抑制率高于姜黄素组和吉西他滨组(P<0.05)。2吉西他滨组、姜黄素组和联合组DNMT1及DNMT3mRNA表达均较对照组降低(P<0.05);联合组低于吉西他滨组和姜黄素组(P<0.05)。3吉西他滨组、姜黄素组和联合组DNMT1蛋白表达均较对照组明显下调(P<0.05,P<0.01),Caspase-3蛋白表达均较对照组明显上调(P<0.01);联合组与吉西他滨组和姜黄素组比较有显著差异(P<0.05)。结论姜黄素可协同吉西他滨促进胰腺癌细胞的凋亡,其机制与调控胰腺癌PANC-1细胞癌基因的去甲基化作用有关。
Objective To investigate the effect of curcumin on gene expression of methylated transferase (DNMTs) in human pancreatic cancer PANC-1 cells and its mechanism. Methods PANC-1 cells cultured in vitro were divided into control group, gemcitabine group, curcumin group and combination group. The expression of DNMTs mRNA was detected by RT-PCR. The expression of DNMT1 and Caspase-3 protein was detected by Western blotting. The expression of DNMT1 mRNA and protein in pancreatic cancer cell line PANC-1 was detected by CCK8 assay. Results 1 The inhibitory rate of PANC-1 cell proliferation in combination group was higher than that in curcumin group and gemcitabine group (P <0.05). The expression of DNMT1 and DNMT3 mRNA in gemcitabine group, curcumin group and combination group were lower than those in control group (P <0.05), and the combination group was lower than gemcitabine group and curcumin group (P <0.05). Compared with control group, the expression of DNMT1 protein in gemcitabine group, curcumin group and combination group were significantly decreased (P <0.05, P <0.01), and Caspase-3 protein expression was significantly increased (P <0.01) Group and curcumin group were significantly different (P <0.05). Conclusions Curcumin may cooperate with gemcitabine in promoting the apoptosis of pancreatic cancer cells. The mechanism may be related to the demethylation of pancreatic cancer PANC-1 cells.