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目的:探讨过氧化氢(H2O2)预处理对人脐带间充质干细胞(hUC-MSCs)损伤的适应性保护作用。方法:HUC-MSCs体外分离培养、扩增纯化、鉴定后绘制生长曲线;对数期hUC-MSCs用不同浓度H2O2作用1h及12h,检测细胞代谢率;对数期hUC-MSCs用10、20、50、100mmo1/L H2O2预处理1h,恢复0h、12h或24h,用700、800、900mmo1/L H2O2损伤12h,观察细胞形态,细胞代谢率及细胞计数。结果:HUC-MSCs分离培养、扩增纯化后鉴定符合其表面标志,且3~6d进入对数期;H2O2诱导hUC-MSCs损伤呈浓度及时间依赖性;10、20、50、100mmo1/L H2O2预处理1h恢复12h减弱700、800、900mmo1/L H2O2作用12h引起的hUC-MSCs损伤。结论:低浓度短时间H2O2预处理对高浓度长时间H2O2引起的hUC-MSCs损伤具有适应性保护作用,且需要适宜的预处理强度及时间间隔。
Objective: To investigate the adaptive protective effect of hydrogen peroxide (H2O2) preconditioning on human umbilical cord mesenchymal stem cells (hUC-MSCs) injury. MSCs were isolated, cultured, expanded, purified and identified. The growth curves of hUC-MSCs were obtained after hUC-MSCs were treated with different concentrations of H2O2 for 1h and 12h. The logarithmic hUC- 50,100mmo1 / L H2O2 pretreatment 1h, recovery 0h, 12h or 24h, 700,800,900mmo1 / L H2O2 damage 12h, observe the cell morphology, cell metabolic rate and cell count. Results: HUC-MSCs were isolated, cultured, identified by their surface markers after amplification and purification, and entered the logarithmic phase from 3 to 6 days. H2O2-induced hUC-MSCs injury in a concentration- and time- Pretreatment 1h recovery 12h and 700,800,900mmo1 / L H2O2 12h induced hUC-MSCs injury. CONCLUSION: Pretreatment with low concentration of H2O2 for a short time has the adaptive protective effect on hUC-MSCs induced by high concentration of H2O2 for a long time and requires appropriate pretreatment intensity and time interval.