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目的:观察氯沙坦对高糖培养人骨骼肌细胞(Human skeletal muscle cells,HSk MCs)中线粒体融合蛋白2(mitofusin2,Mfn2)的表达及其对细胞凋亡的影响。方法:1.使用不同浓度的葡萄糖养基(葡萄糖浓度分别为5.55 mmol/L,11.1 mmol/L,22.2 mmol/L)分别培养HSk MCs细胞株48小时,检测各组细胞中血管紧张素Ⅰ型受体(Angiotensin II type I receptor,AT1R)基因、基因Mfn2的表达,并用流式细胞术检测细胞凋亡。2.根据1中实验结果,选择对Mfn2影响最大的葡萄糖浓度(此组葡萄糖浓度为22.2mmol/L)作为后续实验的条件。加入血管紧张素受体Ⅱ拮抗剂(Angiotensin Receptor Blockers,ARB)氯沙坦(Losartan),处理人骨骼肌细胞(HSk MCs)48 h,以未加氯沙坦为对照组,观察其对线粒体融合蛋白2(Mfn2表达的影响,并行流式细胞术检测细胞凋亡。结果:氯沙坦干预组HSk MCs细胞中Mfn2表达上调,细胞凋亡减少。结论:阻断肾素血管紧张素系统(Renin-angiotensin System,RAS)能上调HSk MCs细胞株中的Mfn2表达,并减少细胞凋亡。
OBJECTIVE: To observe the effect of losartan on the expression of mitofusin2 (Mfn2) in cultured human skeletal muscle cells (HSk MCs) and its effect on apoptosis. Methods: 1. HSk MCs cells were cultured for 48 hours with different concentrations of glucose (5.55 mmol / L glucose, 11.1 mmol / L, 22.2 mmol / L glucose) respectively. The levels of angiotensin type 1 Angiotensin II type I receptor (AT1R) gene and Mfn2 gene were detected by flow cytometry. Apoptosis was detected by flow cytometry. 2. According to the experimental results in 1, select the glucose concentration of Mfn2 most affected (glucose concentration in this group is 22.2mmol / L) as a follow-up experiment conditions. The human skeletal muscle cells (HSK MCs) were treated with losartan (AR) and losartan for 48 h, losartan was used as control group, and mitochondrial fusion was observed (Mfn2) were detected by flow cytometry.Results: The expression of Mfn2 was up-regulated and the apoptosis was decreased in HSK MCs cells treated with Losartan.Conclusion: The renin-angiotensin system (Renin -angiotensin system (RAS) can up-regulate Mfn2 expression in HSk MCs and decrease apoptosis.