论文部分内容阅读
本文着重介绍使用高效液相色谱荧光测定天然食品中维生素B_1、B_2含量。在测试维生素B_1时,使用键合C_(18)分析柱,流动相为CH_3OH-0.01M KH_2PO_4 45/55,流速1.5ml/min,Ex:390nm,EM:470nm,柱温45℃;测维生素B_2时用强阳离子交换柱,流动相CH_3OH/0.01M KH_2PO_4,CH_3OH由30～70%10分钟梯度淋洗,流速1.5ml/min,柱温45℃,Ex:449nm,EM:530nm。在上述条件下所测部分食物中维生素B_1的回收率是92.51～101.85%,变异系数2.14～6.57%;维生素B_2的回收率为89.91～95.18%,变异系数0.19～6.19%。部分食物样品的测试结果令人满意。
This article focuses on the use of high performance liquid chromatography fluorescence determination of natural foods vitamin B_1, B_2 content. In the test of vitamin B_1, the binding C_ (18) analytical column was used, the mobile phase was CH_3OH-0.01M KH_2PO_4 45/55, the flow rate was 1.5ml / min, Ex: 390nm, EM: 470nm, When using a strong cation exchange column, the mobile phase CH_3OH / 0.01M KH_2PO_4 and CH_3OH were eluted with a gradient of 30-70% for 10 minutes at a flow rate of 1.5 ml / min. The column temperature was 45 ° C, Ex: 449 nm and EM: 530 nm. Under the above conditions, the recovery rate of vitamin B_1 in some foods was 92.51 ~ 101.85% and the coefficient of variation was 2.14 ~ 6.57%. The recovery rate of vitamin B_2 was 89.91 ~ 95.18% and the coefficient of variation was 0.19 ~ 6.19%. Some food samples test results are satisfactory.