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将球形芽孢杆菌两毒蛋白基因分别克隆并分别在大肠杆菌及鱼腥藻中表达，经对淡色库蚊幼虫毒性测试结果表明：只表达一种毒蛋白基因的大肠杆菌或鱼腥藻对蚊幼虫无毒效，而将分别表达这两种基因的大肠杆菌或鱼腥藻等浓度混合使用时则表现出高的杀蚊活性，从而证明球形芽孢杆菌两毒蛋白基因具有明显的协同作用，其杀蚊活性与同细胞表达两种毒蛋白基因的大肠杆菌或鱼腥藻相比，结果相似，无明显差异。
The two Bacillus spores protein genes were cloned and respectively expressed in E. coli and Anabaena, Culex pipiens pallens larvae toxicity test results showed that: only expressed a toxic protein gene of E. coli or Anabaena mosquito larvae Non-toxic, and the expression of these two genes respectively Escherichia coli or Anabaena concentration mixed use showed high anti-mosquito activity, which proved that the two Bacillus subtilis protein genes have a significant synergistic effect, which kill The mosquito activity was similar to that of the E. coli or Anabaena that expressed the two toxin genes in the cells, with no significant difference.