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用Ｆｕｒａ－２荧光测定法和ＲＩＡ，对２４例慢性肾功能衰竭（ＣＲＦ）维持血透患者（男１６例，女８例，年龄２６～７１岁，平均５０．９岁）血小板的胞浆游离钙浓度和血栓素Ｂ２（ＴＸＢ２）含量进行了测定。ＣＲＦ组透析前、后血小板胞浆游离钙浓度均高于正常对照组（Ｐ均＜０．００１），透析后低于透析前（Ｐ＜０．００２）；透前、后血小板ＴＸＢ２均低于正常（Ｐ分别小于０．００１和０．０５），透析后高于透析前（Ｐ＜０．００１）。血小板胞浆钙浓度变化与ＴＸＢ２生成不相关。Ｐ＜０．００１：与透析前比，ΔＰ＜０．０５，ΔΔＰ＜０．００２在相关分析上，Ｐｔ［Ｃａ２＋］ｉ与血小板ＴＸＢ２在正常对照组不相关（ｒ＝－０．２７，ｐ＜０．１），透析前、后也均不相关（ｒ＝０．３４和－０．２２，ｐ分别＜０．２和０．５）。讨论Ｃａ２＋是重要的细胞内信使，多种形式的细胞损伤或细胞处于病理生理状态下，常伴有胞浆钙水平的增高。血小板胞浆Ｃａ２＋在刺激信号的转导中发挥重要的作用，促使血小板的变形、聚集、释放，调节血小板的花生四烯酸代谢［３］。有关研究已证实，血小板［Ｃａ２＋］ｉ的变化能诱发内源性花生四烯酸代谢物的释放，参与ＴＸＡ２生成的调节；而ＴＸＡ２对［Ｃａ?
Fura-2 fluorimetric assay and RIA were performed on 24 patients with chronic renal failure (CRF) who maintained hemodialysis (16 males and 8 females, aged 26-71 years with an average of 50.9 years of age) Calcium concentration and thromboxane B2 (TXB2) content were determined. The levels of platelet cytoplasmic free calcium before and after dialysis in CRF group were significantly higher than those in normal control group (all P <0.001), but lower than those before dialysis (P <0.002) Normal (P less than 0.001 and 0.05, respectively) and higher after dialysis than before dialysis (P <0.001). Changes in platelet cytoplasmic calcium concentration and TXB2 generation is not related. P <0.001 vs. pre-dialysis, ΔP <0.05, ΔΔP <0.002 Pt [Ca2 +] i was not associated with platelet TXB2 in the normal controls (r = -0.27, p <0.1), neither before nor after dialysis (r = 0.34 and -0.22, p <0.2 and 0.5, respectively). Discussion Ca2 + is an important intracellular messenger, a variety of forms of cell damage or cells in the pathophysiological state, often accompanied by an increase in cytoplasmic calcium levels. Platelet cytoplasmic Ca2 + plays an important role in the transduction of stimulus signals, which can promote the deformation, aggregation and release of platelets and regulate platelet arachidonic acid metabolism [3]. Studies have confirmed that changes in platelet [Ca2 +] i can induce the release of endogenous arachidonic acid metabolites involved in the regulation of TXA2 production; and TXA2 on [Ca ~