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目的分析河北省脊髓灰质炎(脊灰)实验室监测数据,评估其运转情况,为河北省继续维持无脊灰状态提供实验室数据。方法 分析全省11个市上报的急性弛缓性麻痹(AFP)病例个案送检表、河北省脊灰实验室病原学监测数据和国家脊灰实验室对所有脊灰病毒(PV)株进行型内鉴定数据。结果 2001-2009年河北省脊灰实验室共收到3 422例AFP病例粪便标本,282例AFP病例接触者粪便标本。AFP病例中分离到PV 280例,年均分离率为8.2%;分离到非脊灰肠道病毒(NPEV)368例,年均分离率为10.8%;AFP病例接触者粪便标本中分离出PV 25例,年均分离率为8.9%,分离出NPEV 64例,年均分离率为22.7%。未发现脊灰野病毒或疫苗衍生脊灰病毒(VDPV)。脊灰实验室每年均通过WHO和国家脊灰实验室盲样标本的能力验证和现场认证评估,细胞系敏感性均达到WHO的要求。结论 河北省脊灰实验室运转良好,细胞系敏感、有效,河北省继续保持无脊灰状态。
Objective To analyze the laboratory monitoring data of poliomyelitis (polio) in Hebei Province and assess the operation of the poliovirus in Hebei Province, providing laboratory data for the continuous maintenance of polio-free status in Hebei Province. Methods The cases of acute flaccid paralysis (AFP) reported by 11 municipalities in the province were analyzed. The data of etiological monitoring of poliovirus in Hebei Province and the national poliovirus laboratory Identification data. Results A total of 3 422 fecal samples of AFP cases and 282 fecal samples of AFP cases were collected from the polio laboratory in Hebei Province during 2001-2009. 280 cases were isolated from AFP cases, the average annual isolation rate was 8.2%; 368 cases with non-polio enterovirus (NPEV) were isolated with an average annual isolation rate of 10.8%; PV 25 was isolated from stool samples of AFP cases For example, with an average annual rate of 8.9%, 64 cases of NPEV were isolated with an average annual rate of 22.7%. No poliovirus or vaccine-derived poliovirus (VDPV) was found. Each year, the polio laboratory passes the proficiency testing and on-site certification assessment of blind specimens from WHO and the National Polio Laboratory, and the cell line sensitivity meets the WHO requirements. Conclusion The polio laboratory in Hebei Province runs well and the cell lines are sensitive and effective. Hebei Province continues to maintain a polio-free status.