论文部分内容阅读
目的:通过观察辛二酰苯胺异羟肟酸(Suberoylanilide Hydroxamic Acid,SAHA)对于骨癌痛(cancerinduced bone pain,CIBP)大鼠脊髓内谷氨酸转运体1(glutamate transporter 1,GLT-1)表达的影响,探讨GLT-1参与SAHA镇痛的相关机制。方法:将体重180~220 g雌性SD大鼠随机分为4组:Sham+vehicle组(A)、Sham+SAHA组(B)、CIBP+vehicle组(C)、CIBP+SAHA组(D)。经腹膜腔注射50 mg/kg SAHA,1/d。利用von Frey纤维测量大鼠手术侧机械缩足反射阈值(paw withdrawal threshod,PWT)。上述4组大鼠在不同时间点处死后取其腰膨大段脊髓,应用免疫组织化学染色和Western Blot方法观察大鼠腰膨大节段手术侧脊髓背角内GLT-1的表达情况。结果:与A组相比,C组大鼠PWT自术后第7 d开始显著下降,且一直持续至术后第14 d(P<0.01),脊髓背角内GLT-1水平逐渐下调,呈时间依赖性(P<0.05)。D组与C组相比,PWT升高(P<0.05),术侧脊髓背角内GLT-1表达增加(P<0.05)。结论:腹膜腔注射SAHA能够有效缓解骨癌痛,其部分机制可能与促进脊髓背角内GLT-1的表达有关。
OBJECTIVE: To observe the effect of suberoylanilide hydroxamic acid (SAHA) on the expression of glutamate transporter 1 (GLT-1) in the spinal cord of cancer-induced bone pain (CIBP) To explore the mechanism of GLT-1 involved in SAHA analgesia. Methods: Female Sprague Dawley rats weighing 180-220 g were randomly divided into 4 groups: Sham + vehicle group (A), Sham + SAHA group (B), CIBP + vehicle group (C) and CIBP + SAHA group (D). Peritoneal injection of 50 mg / kg SAHA, 1 / d. Rat paw withdrawal threshod (PWT) was measured using von Frey fibers. The rats in the four groups were sacrificed at different time points to take the lumbar enlargement of the spinal cord. The expression of GLT-1 in the spinal dorsal horn of the lumbar spinal segment was observed by immunohistochemical staining and Western Blot. Results: Compared with group A, PWT in group C decreased significantly from the 7th day after operation until the 14th day after operation (P <0.01), and the level of GLT-1 in spinal dorsal horn decreased gradually Time-dependent (P <0.05). Compared with group C, group D had higher PWT (P <0.05) and increased GLT-1 expression in spinal dorsal horn (P <0.05). Conclusion: Intraperitoneal injection of SAHA can effectively relieve bone cancer pain, and some of its mechanisms may be related to the promotion of GLT-1 expression in spinal dorsal horn.