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目的 :建立测定人血浆中尼索地平的毛细管气相色谱电子捕获检测法。方法 :色谱柱为 2 5m× 0 32 8mmDB- 1交联熔融毛细管柱 ,膜厚 0 5 2 μm ,检测器为63Ni电子捕获检测器。内标为尼莫地平 ,血浆样品在碱性条件下用乙醚 -环己烷 (1∶1)提取。结果 :在 0 1～ 30ng·mL-1浓度范围内峰面积比与浓度呈良好的线性关系 ,r =0 9997。人血浆中尼索地平的最低检出浓度为 5 0pg·mL-1。高、中、低 3种浓度的平均回收率为 91 92 %～ 97 89% ,日内和日间精密度的RSD分别小于 4 6 %和 6 9%。结论 :此方法为尼索地平的药代动力学研究提供一检测方法
Objective: To establish a capillary gas chromatography electron capture method for the determination of nisoldipine in human plasma. METHODS: The column was a 2 5 m × 0 32 8 mm DB-1 cross-linked fused capillary column with a thickness of 522 μm and a detector of 63Ni electron capture detector. The internal standard was nimodipine, and plasma samples were extracted with ether-cyclohexane (1: 1) under basic conditions. Results: There was a good linear relationship between peak area ratio and concentration in the range of 0 1 ~ 30 ng · mL-1, r = 0 9997. The minimum detectable concentration of nisoldipine in human plasma was 50pg · mL-1. The average recoveries of high, medium and low concentrations were 91 92% -97 89%. The intra-day and inter-day RSDs were less than 46% and 69%, respectively. Conclusion: This method provides a test for the pharmacokinetics of nisoldipine