Biomolecular hydration dynamics probed with 2D-IR spectroscopy:From dilute solution to a macromolecu

• 作 者: John T.King Evan J.Arthur Derek G.Osborne Charles L.Brooks Ⅲ Kevin J.Kubarych
• 机 构: Department of Chemistry,University of Michigan,LSA Biophysics,University of Michigan,Department of M
• 出 处: Chinese Chemical Letters
• 发表日期: 2015年04期
• 关键词: hydration dilute crowd vibrational biochemical interfaces environments labeling

Although it is well known that water is essential for biological function,it has been a challenge to determine how water behaves near biomacromolecular interfaces,and what role water plays in influencing the dynamics of the biochemical machinery.By adopting a vibrational labeling strategy coupled with ultrafast two-dimensional infrared(2D-IR) spectroscopy,it has recently become possible to study hydration dynamics,site specifically at the surface of proteins and model membranes.We review our recent progress in measuring hydration dynamics in contexts ranging from small-molecule solutes to biomacromolecules in dilute,viscous,and crowded environments.

Although it is well known that water is essential for biological function, it has been a challenge to determine how water behaves near biomacromolecular interfaces, and what role water plays in influencing the dynamics of the biochemical machinery. By adopting a vibrational labeling strategy coupled with ultrafast two-dimensional infrared (2D-IR) spectroscopy, it has recently become become able to study hydration dynamics, site specifically at the surface of proteins and model membranes. We review our recent progress in measuring hydration dynamics in contexts ranging from small-molecule solutes to biomacromolecules in dilute, viscous, and crowded environments.