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1903年我国植物学家米哈依尔、西蒙诺维奇、茨维特(МихаилСеменовицЦвет)发表了一篇论文:“一种新的吸附现象及其在生物化学分析中的应用”,在这篇论文里叙述了分离叶子里的色素的方法,这个方法作者叫它做色层分离法。茨维特的功绩,除了发现现象本身以外,在于他正确地估计了这个方法的价值;他指出:原则上可以把这个方法用于由具有各种各样的性质的物质——包括没有颜色的物质在内——所组成的复杂混合物的分析和分离。为了分离叶绿素和叶子里其他的色素,茨维特用一个装满吸附剂(碳酸钙,糖或其他的吸附剂)的玻璃管,而使在苯或其他溶剂中的叶子提出物通过玻璃管。
In 1903, Chinese botanists Mikhail, Simonovic and Zvitt published a paper entitled “A new phenomenon of adsorption and its application in biochemical analysis”, in this paper Described the method of separating the pigment in the leaves, the author of this method called the chromatographic separation method. In addition to discovering the phenomenon itself, Zvette’s merit lies in the fact that he correctly estimates the value of this method; he points out that it is in principle possible to apply this method to substances made up of a wide range of substances, including substances without color In - the composition of the complex mixture of analysis and separation. In order to separate the chlorophyll and other pigments in the leaves, Zwickt uses a glass tube filled with adsorbent (calcium carbonate, sugar or other adsorbent) to pass the leaf extract in benzene or other solvent through the glass tube.