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目的:观察有氧运动对高糖高脂膳食大鼠肝组织碳水化合物反应元件结合蛋白(ChREBP)mRNA表达的影响。方法:32只雄性SD大鼠随机分为4组:安静对照组、运动对照组、高糖高脂安静组和高糖高脂运动组,每组8只。安静对照组和运动对照组食用普通饲料,高糖高脂组食用高糖高脂饲料,运动组每天运动1小时,跑速为25m/min,坡度为0°,每周运动6天。6周后,取血检测总甘油三酯(TG)、总胆固醇(TC)浓度,并取附睾与肾周脂肪垫称重,用荧光定量PCR技术检测肝组织ChREBP mRNA表达水平。结果:高糖高脂安静组附睾与肾周脂肪垫重量和血TG、TC浓度均显著高于安静对照组(P<0.05),运动对照组则明显低于安静对照组(P<0.05);与高糖高脂安静组比较,高糖高脂运动组体重和附睾与肾周脂肪垫重量均明显降低(P<0.05),血TG浓度亦显著降低(P<0.01);高糖高脂安静组ChREBP mRNA表达显著高于安静对照组(P<0.01),运动对照组与普通安静组无明显差别,而高糖高脂运动组显著低于安静对照组(P<0.01)。结论:有氧运动能有效抑制高糖高脂膳食引起肝脏组织中ChREBP mRNA表达增加,可能是有氧运动降低高糖高脂膳食造成机体肥胖的机理之一。
Objective: To observe the effect of aerobic exercise on the expression of carbohydrate response element binding protein (ChREBP) mRNA in the liver tissue of rats fed high glucose and high fat diet. Methods: Thirty-two male Sprague-Dawley rats were randomly divided into 4 groups: control group, exercise control group, high-sugar and high-fat group and high-fat and high-fat exercise group. Quiescent control group and exercise control group consumed ordinary feed, high-sugar and high-fat group consumed high-sugar and high-fat diet. The exercise group exercised for 1 hour a day, running speed was 25 m / min, slope 0 °, exercise 6 days per week. Six weeks later, total blood triglyceride (TG) and total cholesterol (TC) were collected for blood test. Epididymal and perirenal fat pads were weighed and the expression of ChREBP mRNA was detected by real-time PCR. Results: The weights of epididymal fat and perirenal fat pad and TG and TC concentrations in high fat and fat tranquilization group were significantly higher than those in quiet control group (P <0.05) and those in control group were significantly lower than those in quiet control group (P <0.05). Compared with the hyperglycemia group, the body weight, epididymal fat pad and perirenal fat pad weight in high glucose and high fat exercise group were significantly lower (P <0.05), blood TG concentration was also significantly lower (P <0.01) The expression of ChREBP mRNA in the high glucose and high fat exercise group was significantly lower than that in the quiet control group (P <0.01). There was no significant difference between the exercise control group and the normal quiet group. CONCLUSION: Aerobic exercise can effectively inhibit the increase of ChREBP mRNA expression in liver tissue induced by high-sugar and high-fat diet, which may be one of the mechanisms by which aerobic exercise can reduce body fat caused by high-sugar and high-fat diet.