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目的研究牛磺酸对苯并(a)芘(B(a)P)致人胚肝细胞(L-02细胞)染色体损伤的保护作用。方法以L-02细胞为靶细胞,按完全随机设计分成5组:(1)空白对照组;(2)溶剂对照组;(3)B(a)P染毒组:设12.5,25,50μmol/L3个剂量染毒细胞;(4)牛磺酸组:设1.0,2.0,4.0mmol/L3个浓度,加入培养体系后继续培养24h;(5)牛磺酸预防组:L-02细胞先以3个不同浓度的牛磺酸预处理24h,再加入25μmol/L的B(a)P培养24h。各组均设3个平行样。收获细胞后用微核实验检测染色体损伤,计算微核率和核分裂指数。结果牛磺酸单独作用于L-02细胞时,与空白对照组比较,中、高浓度牛磺酸诱导的微核率降低,核分裂指数增高,差异有统计学意义。从低到高3个剂量B(a)P单独染毒L-02细胞诱导的微核率为(34.67±2.52),(45.33±4.16),(60.00±7.21)%。核分裂指数分别为1.326±0.034,1.228±0.006,1.148±0.012,与溶剂对照组比较微核率升高,核分裂指数降低,差异有统计学意义,且两者均呈明显的剂量-反应关系。低、中、高浓度牛磺酸预防组诱导的微核率对应为(30.33±3.51),(25.67±1.53)和(23.00±1.00)%。均比单纯25μmol/LB(a)P染毒组诱导的微核率降低,差异有统计学意义。低、中、高浓度牛磺酸预防组对应的核分裂指数为1.455±0.011,1.474±0.015和1.1492±0.013,比单纯25μmol/LB(a)P染毒诱导的核分裂指数高,差异有统计学意义,且两者均呈剂量-反应关系。结论B(a)P能诱导肝细胞染色体损伤,且存在明显的剂量-反应关系;牛磺酸预处理对B(a)P引起的肝细胞毒性具有明显保护作用。
Objective To study the protective effect of taurine on chromosome damage of human embryonic hepatocytes (L-02 cells) induced by benzo (a) pyrene (B (a) P). Methods L-02 cells were randomly divided into five groups according to randomized design: (1) blank control group; (2) solvent control group; (3) B (a) (4) Taurine group: 1.0,2.0,4.0 mmol / L concentration was added into the culture system for 24 h; (5) Taurine prevention group: L-02 cells first Three different concentrations of taurine were pretreated for 24 h and then incubated with 25 μmol / L B (a) P for 24 h. Each group were set 3 parallel samples. After harvested, the chromosome damage was detected by micronucleus test and the micronucleus rate and mitotic index were calculated. Results Compared with the blank control group, the taurine-induced micronucleus rate and mitotic index were increased in the L-02 cells treated with taurine alone. The difference was statistically significant. The micronucleus rates of L-02 cells exposed to 3 doses of B (a) P from low to high were (34.67 ± 2.52), (45.33 ± 4.16) and (60.00 ± 7.21)%, respectively. The mitotic index were 1.326 ± 0.034,1.228 ± 0.006,1.148 ± 0.012, respectively. Compared with the solvent control group, the micronucleus rate and mitotic index decreased, the differences were statistically significant, and both showed a significant dose-response relationship. The micronucleus rates induced by low, middle and high concentrations of taurine prevention group were (30.33 ± 3.51), (25.67 ± 1.53) and (23.00 ± 1.00)%, respectively. Compared with the 25μmol / LB (a) P exposure group, the micronucleus rate was significantly decreased. There was significant difference between the two groups. The mitotic index of low, middle and high concentration of taurine prevention group was 1.455 ± 0.011,1.474 ± 0.015 and 1.1492 ± 0.013, higher than that of 25μmol / LB (a) P group, the difference was statistically significant , And both showed a dose-response relationship. Conclusion B (a) P can induce chromosome damage in hepatocytes, and there is a significant dose-response relationship. Taurine preconditioning has a significant protective effect on hepatotoxicity induced by B (a) P.