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目的 :为了获得人防御素多肽 ,实验构建了防御素基因原核表达载体并使其在大肠杆菌中表达。方法 :采用从pUC18载体上回收HNP 1外源基因片段 ,使其与载体pET30b连接 ,获得pET30b HNP 1重组质粒 ,并在大肠杆菌BL 2 1(DE3)株中表达。结果 :发现人防御素融合蛋白得到表达。结论 :人防御素基因 (HNP 1)可在大肠杆菌BL 2 1(DE3)株中表达 ,目的蛋白在菌体内以包涵体形式存在
OBJECTIVE: To obtain human defensin peptides, a prokaryotic expression vector of defensin gene was constructed and expressed in Escherichia coli. Methods: HNP 1 gene fragment was recovered from pUC18 vector and ligated with vector pET30b to obtain pET30b HNP 1 recombinant plasmid and expressed in E. coli BL21 (DE3) strain. Results: Human defensin fusion protein was found to be expressed. CONCLUSION: The human defensin gene (HNP 1) is expressed in E. coli BL 2 1 (DE3) strain. The target protein exists in the form of inclusion bodies