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目的：以腹腔巨噬细胞（ ＭΦ） Ｉａ 抗原表达阳性 率和抗体依赖细胞 介导的细 胞毒作用（ Ａ Ｄ Ｃ Ｃ） 为指标，观察六味地黄 汤及其拆 方对大鼠 腹腔 ＭΦ 功能 的调节作 用。方法： Ｉａ 抗原 表达的检测 用 Ａ Ｐ Ａ Ａ Ｐ 桥 联酶标免疫测试方法； Ａ Ｄ Ｃ Ｃ 测定用孔雀绿比色法。结果：六味地黄 汤能明显 提高正常 大鼠腹腔 ＭΦ表 面 Ｉａ 抗原 表达的阳性率及 Ａ Ｄ Ｃ Ｃ 的细 胞毒指数，还能够使皮质酮（ Ｃ Ｏ Ｒ Ｔ） 肌注所致“阴虚”模型 大鼠 ＭΦ Ｉａ 抗 原表达率 的抑制及 Ａ Ｄ Ｃ Ｃ 细胞 毒活性的降低得以明显改善。三补方能提高正常大鼠腹腔 ＭΦ 表面 Ｉａ 抗原表 达的阳性 率及 Ａ Ｄ Ｃ Ｃ 的细胞毒指数，并改善“阴虚”大鼠 ＭΦ Ｉａ 抗原表达率的抑制及 Ａ Ｄ Ｃ Ｃ 细胞毒活性的降低，而三泻方对其影响不明显。结论：六味地黄汤对 ＭΦ功能具有明显的调节 作用，三味补药是 该方影响 ＭΦ Ｉａ 抗原表 达及 Ａ Ｄ Ｃ Ｃ 活性的主要成分，三味泻药对正常 及模 型大 鼠的 ＭΦ 功能虽 无明 显影 响， 但与 三补 配伍 后 则能 够明 显加 强 其对 ＭΦ Ｉａ 抗原表达阳性率及 Ａ Ｄ Ｃ Ｃ 活性的作用，体现了三补与三泻配伍的协同和增效 作用。
Objective: To observe the effect of Liuwei Dihuang Decoction and its decomposed prescriptions on the function of abdominal peritoneal MΦ in rats by using the positive rate of peritoneal macrophage (MΦ) Ia antigen expression and antibody-dependent cell-mediated cytotoxicity (A D C C) as indicators. effect. Methods: Detection of Ia antigen expression was performed using the A P A A P bridge enzyme immunoassay method; A D C C assay using the malachite green colorimetric method. RESULTS: Liuwei Dihuang Decoction can significantly increase the positive rate of Ia antigen expression on the surface of normal peritoneal MΦ and the cytotoxicity index of A D C C, and can also cause the “yin deficiency” model caused by intramuscular injection of corticosterone (C O R T). The inhibition of the expression of the rat MΦ Ia antigen and the decrease of A D C C cytotoxicity were significantly improved. The three supplements can increase the positive rate of Ip antigen expression on the surface of normal peritoneal MΦ and the cytotoxicity index of A D C C , and improve the inhibition of the expression of MΦ Ia antigen in rats with Yin deficiency and A D C C cytotoxicity. The decrease of activity, but the effect of San Xie Fang is not obvious. Conclusion: Liuwei Dihuang Decoction has obvious regulation effect on the function of MΦ, and Sanwei tonic is the main component that affects the expression of MΦ Ia antigen and A D C C activity. Sanwei laxative has no obvious effect on the function of MΦ in normal and model rats. However, after compatibility with Sanbu, the effect on the positive expression rate of MΦ Ia antigen and A D C C activity can be significantly enhanced, reflecting the synergistic and synergistic effects of compatibility between Sanbu and Sanxia.