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目的研究灵丹菌质的遗传毒性,为其开发利用提供依据。方法利用Ames试验、小鼠精子畸形试验以及骨髓细胞微核试验评价灵丹菌质的遗传毒性。Ames试验设立8、40、200、1 000和5 000μg/皿5个剂量组,计算各剂量组在加与不加体外代谢活化系统S9的情况下,对鼠伤寒沙门氏菌TA97、TA98、TA100和TA102的诱发回变菌落数。小鼠精子畸形试验设5、10和20 ml/kg·BW 3个剂量组,计算各剂量组小鼠精子畸形率。小鼠骨髓细胞微核设5、10和20 ml/kg·BW 3个剂量组计算各剂量组微核发生率。结果受试物各剂量组4种菌株的回变菌落数均未超过未处理对照组的2倍;受试物各剂量组的精子畸形率和微核细胞率均未见统计学意义。结论在本研究条件下,灵丹菌质未见遗传毒性。
Objective To study the genotoxicity of Cladophora bifidus and provide the basis for its development and utilization. Methods Ames test, mouse sperm abnormality test and bone marrow micronucleus test were used to evaluate the genotoxicity of Cladosporium. Ames test set up 8, 40, 200, 1 000 and 5 000 μg / dish of 5 dose groups, calculate each dose group with and without the addition of metabolic activation system S9 case, the Salmonella typhimurium TA97, TA98, TA100 and TA102 Induced back to change the number of colonies. Mouse sperm deformity test set 5, 10 and 20 ml / kg · BW three dose groups, calculate the sperm deformity rate of mice in each dose group. Micronucleus of mouse bone marrow cells were set at 5, 10 and 20 ml / kg · BW. The micronuclei incidence of each dose group was calculated. Results The numbers of colonies returned by the four strains in each dose group did not exceed twice that of the untreated control group. The sperm deformity rate and the rate of micronuclei in each dose group did not reach statistical significance. Conclusion Under the conditions of this study, genistein did not show genotoxicity.