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目的：探讨载脂蛋白（Ａｐｏ）Ｅ基因多态性与冠心病的相关性，以及ＡｐｏＥ基因多态性对冠心病患者血脂水平的影响。方法：１００例冠心病患者和４３例正常对照者。按常规方法测定血浆脂质和Ａｐｏ水平。ＡｐｏＥ基因型的确定采用聚合酶链反应和ＨｈａＩ酶切的方法。结果：本研究只发现３种常见的ＡｐｏＥ基因型，即Ｅ３／３，Ｅ３／４，Ｅ３／２。在病例组和正常对照组之间，ＡｐｏＥ等位基因频率以及基因型频率分布没有统计学差异（Ｐ＞０．０５）。在冠心病患者不同基因型之间，总胆固醇和ＡｐｏＢ的水平有差异（Ｐ＜０．０５）；其它血脂指标无差异（Ｐ＞０．０５）。结论：本研究证实ＡｐｏＥ基因多态性影响冠心病患者总胆固醇和ＡｐｏＢ的水平；但ＡｐｏＥ基因多态性并不是冠心病发病的直接危险因子。
Objective: To investigate the association between apolipoprotein (Apo) E gene polymorphism and coronary heart disease and the effect of ApoE gene polymorphism on serum lipid levels in patients with coronary heart disease. Methods: 100 patients with coronary heart disease and 43 normal controls. Plasma lipid and Apo levels were determined routinely. ApoE genotypes were determined by polymerase chain reaction and HhaI digestion. RESULTS: Three common ApoE genotypes were found in this study, namely E3 / 3, E3 / 4 and E3 / 2. There was no significant difference in ApoE allele frequency and genotype frequency between the case group and the normal control group (P> 0.05). The levels of total cholesterol and ApoB were different between different genotypes of patients with coronary heart disease (P <0.05). There was no significant difference in other lipids (P> 0.05). Conclusion: This study confirmed that ApoE gene polymorphism affects the level of total cholesterol and ApoB in patients with coronary heart disease. However, ApoE gene polymorphism is not a direct risk factor for coronary heart disease.