论文部分内容阅读
目的探讨脆性组氨酸三联体(FHIT)基因在胆管癌细胞株QBC939中的表达及其对细胞生物学行为的影响。方法将构建的FHIT真核表达质粒转染入QBC939细胞,采用荧光定量RT-PCR检测转染前后FHIT基因表达的变化,并采用光镜直接计数法观察转染前后细胞生物学行为的变化。结果转染后QBC939细胞的FHIT基因表达提高了2.53倍,细胞克隆数量明显下降[(11.2±1.3)vs.(10.5±1.1)vs.(6.3±1.0),P<0.05]。结论FHIT基因在QBC939细胞中表达减弱,恢复表达后能抑制细胞克隆形成。
Objective To investigate the expression of fragile histidine triad (FHIT) gene in human cholangiocarcinoma cell line QBC939 and its effect on cell biology. Methods The constructed FHIT eukaryotic expression plasmid was transfected into QBC939 cells. Fluorescent quantitative RT-PCR was used to detect the expression of FHIT gene before and after transfection. The changes of cell biological behavior before and after transfection were observed by light microscope. Results The expression of FHIT gene in QBC939 cells increased 2.53-fold after transfection and the number of cell clones decreased significantly ([11.2 ± 1.3] vs. (10.5 ± 1.1) vs. (6.3 ± 1.0), P <0.05]. Conclusion The expression of FHIT gene is weakened in QBC939 cells, and the expression of FHIT gene can inhibit the cell clone formation.