论文部分内容阅读
目的探讨黄芪注射液对大鼠脑缺血再灌注损伤的神经保护作用和机制。方法成年健康雄性Wistar大鼠70只,应用线栓法经左侧颈外-颈内动脉插线建立大脑中动脉阻塞再灌注模型,经腹腔注射1g/L黄芪注射液(3ml/kg)干预治疗。Longa法评价大鼠神经功能缺损程度,氯化三苯基四氮唑(TTC)染色观察脑梗死体积,苏木素-伊红染色观察顶叶皮质区神经元形态结构变化,透射电子显微镜观察神经细胞的超微结构,流式细胞术检测细胞凋亡,Western blotting检测c-Jun氨基末端激酶3(JNK3)蛋白表达,RT-PCR检测JNK3 mRNA表达。结果经黄芪注射液治疗后,大鼠顶叶皮质区神经元JNK3 mRNA和JNK3蛋白表达较对照组显著减低,凋亡细胞数量显著减少,脑梗死体积显著缩小,神经元形态和超微结构以及动物神经行为功能显著改善。结论黄芪注射液可能通过下调神经元JNK3基因表达而抑制细胞凋亡,缩小脑梗死体积,改善动物的神经行为功能。
Objective To investigate the neuroprotective effect and mechanism of Astragalus injection on cerebral ischemia-reperfusion injury in rats. Methods Seventy male Wistar rats were randomly divided into three groups: the middle cerebral artery occlusion and reperfusion model was established by inserting the left external carotid artery-internal carotid artery by thread plug method, and the intra-abdominal injection of 1g / L astragalus injection (3ml / kg) . Longa method was used to evaluate the degree of neurological deficit in rats. The volume of cerebral infarction was observed by TTC staining. Morphological changes of neurons in parietal cortex were observed by hematoxylin-eosin staining. Transmission electron microscopy The ultrastructure and apoptosis of cells were detected by flow cytometry. The protein expression of c-Jun N-terminal kinase 3 (JNK3) was detected by Western blotting. The expression of JNK3 mRNA was detected by RT-PCR. Results After astragalus injection, the expression of JNK3 mRNA and JNK3 protein in parietal cortex of rats decreased significantly compared with the control group, the number of apoptotic cells was significantly reduced, the volume of cerebral infarction was significantly reduced, the morphology and ultrastructure of neurons and animals Neurobehavioral function improved significantly. Conclusion Astragalus injection may inhibit the apoptosis, reduce the volume of cerebral infarction and improve the neurobehavioral function of the animals by down-regulating the expression of JNK3 in neurons.