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在肺结核的治疗中,现普遍改用一天一次口服异菸肼(INH)0.3克的方案及其它间歇疗法。为了配合临床用药,我们建立了血中INH的化学分析法,为研究血药动力学提供一个有力的手段。本方法是将1毫升血清或血浆置于玻璃离心管内,加1％ZnSO_41毫升,蒸馏水7毫升混匀,5分钟后加0.57NNaOH1毫升摇匀离心10分钟,吸出上层清旅,记上体积,加入5％EDTA(依地酸)0.1毫升,混匀后用稀碱调PH至中性,然后慢慢加入预先准备好的弱阳离子交换树脂层析柱内(Amberlite,C G 50,11,200～400,树脂呈H型,用蒸镏水洗至PH中性,内径0.5cm,高5cm),控制流速为每分钟0.2毫升左右,待样品流完后,加入10毫升蒸馏水洗去吸附在树脂上的杂质和异菸肼代
In the treatment of tuberculosis, the regimen of once daily oral administration of isoniazid (INH) (0.3 g) and other intermittent therapies are now commonly used. In order to cooperate with the clinical medication, we have established a chemical assay of INH in blood, which provides a powerful tool for studying the pharmacokinetics of blood. The method is to place 1 ml of serum or plasma in a glass centrifuge tube, add 1% ZnSO 4 ml and distilled water 7 ml and mix for 5 minutes, then add 0.57N NaOH to 1 ml and shake for 10 minutes to aspirate the supernatant broth, record the volume, 0.1 ml of 5% EDTA (edetic acid), mix and dilute to neutral with dilute alkali, and slowly add to a pre-prepared weak cation exchange resin column (Amberlite, CG 50, 11 200 ~ 400, resin Was H-type, washed with distilled water until PH neutral, inner diameter 0.5cm, height 5cm), the control flow rate of about 0.2 ml per minute until the end of the sample flow, add 10 ml of distilled water to remove impurities adsorbed on the resin and the difference Hydrazide generation