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目的探究神经调节蛋白-1(Neuregulin-1,NRG-1)自分泌对心脏微血管内皮细胞功能的影响。方法体外培养人心脏微血管内皮细胞(HCMECs),通过转染NRG-1 shRNA慢病毒颗粒抑制NRG-1的表达,检测其对HCMECs增殖、迁移和抗损伤能力的影响。结果与对照组相比,当NRG-1的自分泌作用被抑制后,HCMECs的增殖和迁移能力显著下降,在缺氧/复氧损伤下,细胞凋亡增加,细胞存活率明显下降;外源性补充重组人NRG-1(rhNRG-1)能进一步加强该作用。结论 NRG-1自分泌能显著促进HCMECs增殖、迁移和抗损伤能力,外源性补充rhNRG-1可进一步加强该作用。
Objective To investigate the effect of neuregulin-1 (NRG-1) autocrine on the function of cardiac microvascular endothelial cells. Methods Human cardiac microvascular endothelial cells (HCMECs) were cultured in vitro. The expression of NRG-1 was inhibited by transfecting NRG-1 shRNA lentivirus into HCMECs. The effects of NRG-1 on the proliferation, migration and anti-injury of HCMECs were detected. Results Compared with the control group, when the autocrine function of NRG-1 was inhibited, the ability of HCMECs to proliferate and migrate significantly decreased. Under hypoxia / reoxygenation injury, cell apoptosis increased and the cell survival rate decreased significantly. Sexually supplemented recombinant human NRG-1 (rhNRG-1) can further potentiate this effect. Conclusion Autocrine of NRG-1 can significantly promote the proliferation, migration and anti-injury of HCMECs. Exogenous rhNRG-1 supplementation can further enhance this effect.