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目的心肌缺血/再灌注早期给予L-精氨酸,观察其对心脏的保护作用。方法雄性Wistar大鼠45只,随机分为3组(n=15):伪手术组(Sham组),缺血/再灌注+生理盐水组(Vehicle组),L-精氨酸早期处理组(L-Arg组)。结扎大鼠心脏左冠脉前降支,建立在体心肌缺血/再灌注模型,缺血30 min、再灌注5 h,分别在缺血/再灌注早期和晚期给予L-精氨酸。动态监测大鼠左心室功能;再灌注结束后,以Evan′s蓝和TTC双染法检测心梗面积,化学发光法检测总NO(NOx)含量,以ELISA法检测心肌组织过氧亚硝基(ONOO-)标志物硝基酪氨酸含量。结果再灌注早期给予L-精氨酸显著减轻了缺血/再灌注诱导的大鼠心功能降低(LVSP和dp/dtmax)、减少了心肌梗死面积、减少了心肌组织细胞毒性物质ONOO-的生成。结论心肌缺血/再灌注早期给予L-精氨酸可以通过增加NO的生成,减轻再灌注诱导的心肌损伤,进而加快心肌缺血/再灌注后心功能的恢复。
Purpose L-arginine is given in the early stage of myocardial ischemia / reperfusion to observe its protective effect on the heart. Methods Forty-five male Wistar rats were randomly divided into 3 groups (n = 15): sham operation group, ischemia / reperfusion + vehicle group, L-arginine early treatment group L-Arg group). The anterior descending coronary artery of the left coronary artery was ligated in a rat model of myocardial ischemia / reperfusion. After ischemia 30 min and reperfusion 5 h, L-arginine was given in early and late ischemia / reperfusion respectively. Left ventricular function was dynamically monitored in rats; myocardial infarction area was detected by Evan’s blue and TTC double staining after reperfusion, and the content of total NO (NO) was detected by chemiluminescence method. The levels of peroxynitrite (ONOO-) marker Nitrotyrosine content. Results L-arginine at early reperfusion significantly reduced myocardial ischemia-reperfusion-induced loss of cardiac function (LVSP and dp / dtmax), reduced myocardial infarct size, and decreased generation of ONOO-, a cardiac myocyte cytotoxic substance . Conclusions L-arginine can reduce the myocardial injury induced by reperfusion and increase the recovery of cardiac function after myocardial ischemia / reperfusion by increasing the production of NO.