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已知小麦红种皮基因与耐收获前发芽有关。作者开发了提取红种皮基因控制的种皮色素方法。本研究中应用的小麦品种在化学分析前进行了红种皮基因分析。Ｎｏｒｉｎ６６和Ｆｕｋｕｗａｓｅｋｏｍｕｇｉ的基因型鉴定为ｒ＿１、Ｒ＿２、ｒ＿３；Ｎｏｒｉｎ１０和Ａｓａｋａｚｅｋｏｍｕｇｉ鉴定为Ｒ＿１、Ｒ＿２、ｒ＿３；Ｋｉｔａｍｉｋｏｍｕｇｉ、Ｆｕｋｕｈｏｍｕｇｉ和Ｎｏｒｉｎ６１分别鉴定为Ｒ＿１、ｒ＿２、Ｒ＿３，ｒ＿１、Ｒ＿２、Ｒ＿３和Ｒ＿１、Ｒ＿２、Ｒ＿３。１２个品种（９个红种皮，３个白种皮）提取了种皮色素。碾磨种子且用乙烷、丙酮和乙醚清洗备好的麸皮以去掉类胡萝卜色素。脱素的麸皮与氢氧化钠一起加热（１００℃）３０ｍｉｎ。将溶液的ｐＨ调到酸性条件以后用ｎ－丁醇提取色素。以３８０纳米到５５０纳米区域测定了醇提取的吸光度。随着红种皮基因的增加吸光度提高。
It is known that the wheat red seed coat genes are related to the resistance to pre-harvest germination. The authors developed a method for extracting the seed coat pigment from red seed coat. The wheat cultivars used in this study were analyzed for the red seed coat protein prior to chemical analysis. The genotypes of Norin66 and Fukuwasekomugi were identified as r_1, r_2, r_3, norin10 and Asakazekomugi as R_1, R_2, r_3, Kitamikomugi, Fukuhomugi and Norin61 as R_1, r_2, R_3, r_1, R_2, R_3 and R_1, R_2, R_3 Cocoa pigment was extracted from 12 cultivars (9 red seed hides and 3 white hides). The seeds are ground and the prepared bran is washed with ethane, acetone and ether to remove the carotenoids. The de-fermented bran heated with sodium hydroxide (100 ° C) for 30 min. After adjusting the pH of the solution to acidic conditions, the pigment was extracted with n-butanol. The absorbance of the alcohol extraction was measured in the region of 380 nm to 550 nm. Absorbance increased with increasing red coat gene.