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采用8种限制性内切酶与247个已定位到水稻12条染色体上的分子探针组合,对典型籼稻(Oryza sativa L.ssp.indica)“南京11”和粳稻(O.sativa L.ssp.japonica)“金南凤M”以及中间型品种“Bellemont”之间存在的零等位位点进行了分析,9个探针揭示出零等位位点的存在。其中,RG573、G122、G93、G402和G1318特异揭示了“南京11”的零等位,G261特异揭示了“金南凤M”的零等位,RG229特异揭示了“Bellemont”的零等位,C397揭示了“南京11”和“Bellemont”的零等位,而RG131揭示了“金南凤M”和“Bellemont”的零等位。利用“南京11”/“金南凤M”的杂交F_2群体,对其中58个单株进行了7个零等位RFLP标记及程氏指数法中的6个形态性状的测验,据此计算出各植株的零等位基因型及籼、粳分类指数,结果表明:零等位标记在F_2群体中呈显性遗传。在F_2群体中进一步对G93、G122、G261、G402、G1318、C397、RG573零等位标记与程氏指数之间进行了成组数据t测验,发现G93、G122、G402、G1318、G397差异达显著或极显著水平,G261、RG573的t值也较大,显示出零等位标记在籼、粳交后代,可特异鉴定籼、粳差异。
Eight restriction enzymes were used in combination with 247 molecular probes that mapped to 12 chromosomes in rice. Oryza sativa L.ssp. Indica “Nanjing 11” and japonica (O.sativa L.ssp .japonica) “Jin Nanfeng M” and the intermediate variety “Bellemont” were analyzed for zero alleles and nine probes revealed the presence of zero alleles. Among them, RG573, G122, G93, G402 and G1318 specifically revealed the zero allele of “Nanjing 11”. G261 specifically revealed the zero allele of “Jinnanfeng M”. RG229 specifically revealed the zero allele of “Bellemont” and C397 revealed The zero digits of “Nanjing 11” and “Bellemont”, while RG 131 revealed the zero digits of “Jin Nan Feng M” and “Bellemont.” Seven crosses of F2 loci in “Nanjing 11” and “Jinnanfeng M” were used to test the seven null loci RFLP markers and the six morphological traits in the Cheng’s index method. Based on these results, The results showed that the zero allele was dominantly inherited in F 2 population. In group F_2, we further conducted a group t test between the zero-allele markers of G93, G122, G261, G402, G1318, C397 and RG573 and Cheng’s index and found that there was a significant difference between G93, G122, G402, G1318 and G397 Or extremely significant level. The values of t of G261 and RG573 were also larger, indicating that the zero-allele markers could be used to identify indica and japonica differentials in indica and japonica offspring.