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Aim To synthetize 6-chloro-8-fluoro-4-methylumbelliferone phosphate(CF-MUP) and utilize it to analyze acid phosphatase activity.Method CF-MUP obtained by phosphorylation of CFMU(6-chloro-8-fluoro-4-methylumbelliferone) which was prepared by Pechmann reaction between 4 and ethyl acetoacetate in the presence of methanesulfonic acid.And then,the utility of CF-MUP as a substrate for acid phosphatase was compared with MUP(4-methylumbelliferone phosphate).Results The new coumarin phosphate,CF-MUP,demonstrated much higher sensitivity and was more robust for detecting the activity of acid phosphatase than MUP in the physiological pH range.Conclusion This new fluorescence dye,CF-MUP,is a convenient tool for the continuous assay of acid phosphatases with a wide range of pH.
Aim To Synthetize 6-chloro-8-fluoro-4-methylumbelliferone phosphate (CF-MUP) and utilized it to analyze acid phosphatase activity. Method CF-MUP obtained by phosphorylation of CFMU (6-chloro-8-fluoro-4-methylumbelliferone ) which was prepared by Pechmann reaction between 4 and ethyl acetoacetate in the presence of methanesulfonic acid. And then then the utility of CF-MUP as a substrate for acid phosphatase was compared with MUP (4-methylumbelliferone phosphate). Results The new coumarin phosphate , CF-MUP, demonstrated much higher sensitivity and was more robust for detecting the activity of acid phosphatase than MUP in the physiological pH range. Contact This new fluorescence dye, CF-MUP, is a convenient tool for the continuous assay of acid phosphatases with a wide range of pH.