论文部分内容阅读
目的:研究左旋四氢巴马汀(l-THP)对L-钙电流的作用。方法:用全细胞膜片箝记录心室肌细胞的L-钙电流。结果:l-THP 1-100μmol·L~(-1)将I_(Ca~(2+)-max)从(999±93)PA分别减少到(700±111)pA(582±66)pA和(420±112)pA(n=6,P<0.01)。l-THP对钙通道有紧张性阻滞作用及使用依赖性。l-THP可将钙通道的恢复时间从(94±39)ms延长到(170±42)ms(n=6,P<0.01)。结论:l-THP对L-钙通道有阻滞作用。
Objective: To investigate the effect of L-tetrahydropalmatine (L-THP) on L-type calcium currents. Methods: Whole-cell patch-clamp was used to record the L-type calcium current in ventricular myocytes. Results: The I (Ca ~ (2 +) - max decreased from (999 ± 93) PA to (700 ± 111) pA (582 ± 66) pA and (420 ± 112) pA (n = 6, P <0.01). L-THP calcium channel has a tense role in the use of retardation and dependence. l-THP prolonged calcium channel recovery from (94 ± 39) ms to (170 ± 42) ms (n = 6, P <0.01). Conclusion: L-THP has a block effect on L-calcium channel.