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目的了解广东省手足口病病例及其密切接触者病原体基因型,为科学防控手足口病提供依据。方法采集手足口病病例及其密切接触者的粪便标本,采用荧光定量PCR检测病原体基因,采用RDa细胞进行病毒分离,对毒株进行VP1区全序列分析。结果 EV71或CA16感染病例密切接触者均分别检出EV71或CA16,阳性率分别为25.64%和38.03%;EV71毒株与C4亚型的C4a群同源性最高(98.5%~98.7%);5株CA16毒株与B1亚型的B1b群同源性最高(94.9%~97.1%);16株CA16毒株与B1亚型的B1a群同源性最高(97.4%~98.1%);病例与其密切接触者的EV71或CA16毒株核苷酸同源性分别为99.9%~100.0%和99.8%~100.0%。结论 2010年广东省手足口病病原体EV71属C4亚型C4a群;CA16属B1亚型,包括B1a和B1b两个群;病例与其密切接触者病毒分离株的VP1区核苷酸高度同源,但存在变异现象。
Objective To understand the cases of HFMD in Guangdong Province and the pathogen genotypes of their close contacts to provide the basis for scientific prevention and control of HFMD. Methods The faecal specimens of hand, foot and mouth disease and their close contacts were collected. The pathogen genes were detected by real-time fluorescence quantitative PCR. RDa cells were used for virus isolation. The full-length VP1 region was sequenced. Results The positive rates of EV71 or CA16 were detected in EV71 or CA16 cases with positive rates of 25.64% and 38.03%, respectively. The highest homology (98.5% ~ 98.7%) of EV71 or C4 subtype C4a was found The highest homology (94.9% -97.1%) between strain B1 and strain B1 was found between strains CA16 and B1. The homology between strains CA16 and B1 B1a (97.4% -98.1%) was the highest Nucleotide homology of contacts of EV71 or CA16 strains were 99.9% -100.0% and 99.8% -100.0%, respectively. CONCLUSIONS: EV71 is a member of the C4 subtype C4a of HFMD in Guangdong province in 2010. CA16 belongs to the B1 subtype and includes two B1a and B1b populations. The cases are highly homologous to the VP1 nucleotide of the virus isolates in close contact with each other, There is variation.