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目的:研究海参极性脂质对东莨菪碱模型小鼠学习记忆能力的影响,探讨其可能的作用机制。方法:将小鼠随机分为4组:正常对照组、东莨菪碱模型组、海参磷脂(SCP)组和海参脑苷脂(SCC)组。各组小鼠喂食正常饲料,SCP组添加0.5%SCP,SCC组添加0.05%SCC,连续喂养7 d。于第8天造模,正常对照组灌胃生理盐水,其余各组腹腔注射5 mg/kg东莨菪碱。采用Morris水迷宫实验测试小鼠的学习记忆能力,连续7 d。行为测试结束后,对小鼠脑组织进行超氧化物歧化酶(SOD)、丙二醛(MDA)、乙酰胆碱酯酶(TChe)测定。结果:Morris水迷宫实验中,与模型组小鼠相比,SCP和SCC组小鼠潜伏期均缩短,总路程减少,目标象限停留时间比例增加,穿越平台次数增加;SCP和SCC组小鼠脑组织TChe和MDA相对于模型组明显降低,SOD明显增加。结论:海参极性脂质对东莨菪碱致痴呆小鼠的学习记忆能力具有一定的改善作用,其作用机制可能与抗氧化和提高胆碱能有关。
Objective: To study the influence of sea cucumber polar lipid on learning and memory ability of scopolamine model mice and to explore its possible mechanism. Methods: The mice were randomly divided into 4 groups: normal control group, scopolamine model group, sea cucumber phospholipid (SCP) group and sea cucumber cerebroside (SCC) group. Each group of mice fed normal diet, SCP group added 0.5% SCP, SCC group added 0.05% SCC, continuous feeding for 7 days. The model was made on the 8th day. The normal control group was given normal saline, and the rest were intraperitoneally injected with scopolamine 5 mg / kg. The learning and memory ability of mice was tested by Morris water maze test for 7 days. After the behavioral test, the contents of superoxide dismutase (SOD), malondialdehyde (MDA) and acetylcholinesterase (TChe) in the brain of mice were measured. Results: In the Morris water maze test, compared with the model group mice, the latency of the mice in SCP and SCC groups was shortened, the total distance was reduced, the target quadrant retention time was increased and the number of crossing the platform was increased. In the SCP and SCC groups, TChe and MDA relative to the model group decreased significantly, SOD increased significantly. Conclusion: The sea cucumber polar lipids can improve the learning and memory ability of scopolamine-induced dementia mice. The mechanism may be related to the antioxidation and cholinergic function.