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微丝蚴分离技术,1974年以来国外已陆续报告,鉴于近代在寄生虫学方面由于生物化学,免疫学的进展,根据实验研究工作的需要,我们参照文献报道进行了改进与简化,分离出纯净的活微丝蚴。 材料和方法 1 聚蔗糖(Ficoll-400)梯度离心分离法(长爪沙鼠腹腔液中微丝蚴分离法) 1.1 聚蔗糖溶液 Ficoll,分子量400 000 Ty-pe 400(Sigma)以生理盐水或PBS配制Ficoll溶液,使其浓度为10％。 1.2 微丝蚴的分离 取5ml 10％Ficoll溶液置10ml锥形离心管中备用。用腹腔灌洗法,从感染
Microfilariae separation technology has been reported abroad since 1974. In view of the progress of biochemistry and immunology in the field of parasitology in modern times, according to the need of experimental research, we made some improvements and simplifications according to the literatures, and separated the pure Live microfilariae. Materials and Methods 1 Ficoll-400 Gradient Centrifugation (microfilariae separation method in peritoneal cavity of Meriones unguiculatus) 1.1 Ficoll Ficoll with a molecular weight of 400 000 Ty-pe 400 (Sigma) in normal saline or PBS Ficoll solution was prepared to a concentration of 10%. 1.2 microfilariae separation Take 5ml 10% Ficoll solution set 10ml conical centrifuge tube spare. With peritoneal lavage, from infection