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作者从链丝菌S-885的发酵液中分离出一个新的抗病毒抗菌素——9-甲基链霉戊二酰亚胺(9-Methylstreptimidone)(Ⅰ),对脊髓灰白质炎病毒、水疱性口炎病毒和新城鸡瘟病毒有作用。本文报导了它的分离,纯化,理化性质和生物学性质。链丝菌S 885在含有甘油1.0%,淀粉3.0%,蛋白胨1.0%,肉浸膏0.5%,NaCl 0.5%和CaCO_30.2%,pH7.0的培养基中,27℃振荡发酵5天。发酵液滤去菌丝,清液调节pH5.0,用乙酸乙酯提取,乙酸乙酯提出液经水洗及用无水硫酸钠干燥后,减压浓缩至棕褐色糖浆状,溶于苯,经硅胶柱层析,用苯和乙酸乙酯混合液(1∶1)展层、洗脱、蒸干。所得
The authors isolated a new antiviral antibiotic, 9-Methylstreptimidone (I), from the fermentation broth of Streptomyces sp. S-885, against the myeloarthritis virus, blisters Stomatitis virus and Newcastle disease virus play a role. This article reports its separation, purification, physical and chemical properties and biological properties. Streptoverticilli S 885 was fermented in a medium containing glycerol 1.0%, starch 3.0%, peptone 1.0%, meat extract 0.5%, NaCl 0.5% and CaCO 3 0.2%, pH 7.0 for 5 days at 27 ° C with shaking. The broth was filtered to remove mycelium, the supernatant was adjusted to pH5.0, extracted with ethyl acetate, ethyl acetate was washed with water and dried over anhydrous sodium sulfate, concentrated under reduced pressure to a brown syrup, dissolved in benzene, Silica gel column chromatography, using benzene and ethyl acetate mixture (1: 1) spreading, elution, evaporated to dryness. Income