论文部分内容阅读
应用逆转录聚合酶链反应（ＲＴ－ＰＣＲ）技术检测了３４例大肠肿瘤及其相应正常粘膜中ＤＣＣ基因ｍＲＮＡ表达情况。结果：５例大肠腺瘤和２９例大肠癌组织中分别有１例（２０％）、１５例（５１．７％）ＤＣＣ基因ｍＲ－ＮＡ表达发生缺失。其中高、中、低分化的大肠癌组织中该基因ｍＲＮＡ表达缺失率分别为２５．０％（２／８）、４４．４％（４／９）、７５．０％（９／１２）（Ｐ＜０．０５）。而有转移者为７６．９％（１０／１３），无转移者３１．２％（Ｐ＝０．０１８）。ＤＣＣ基因ｍＲＮＡ表达缺失与性别、肿瘤大小及部位无关。提示：对大肠癌原发病灶ＤＣＣ基因ｍＲＮＡ表达的检测将有助于识别高度恶性的大肠癌及判断大肠癌的转移潜能。
Reverse transcription polymerase chain reaction (RT-PCR) was used to detect DCC gene mRNA expression in 34 cases of colorectal tumors and their corresponding normal mucosa. Results: In 5 cases of colorectal adenoma and 29 cases of colorectal cancer, 1 case (20%) and 15 cases (51.7%) of the DCR gene mR-NA were deleted. Among the high, moderate and poorly differentiated colorectal cancer tissues, the gene mRNA deletion rate was 25.0% (2/8), 44.4% (4/9), and 75.0% (9/12), respectively ( P<0.05). There were 76.9% (10/13) of metastatic patients and 31.2% of non-metastatic patients (P=0.018). The absence of DCC gene mRNA expression was not related to sex, tumor size and location. Tip: The detection of DCC gene mRNA expression in colorectal cancer primary lesions will help identify highly malignant colorectal cancer and determine the metastatic potential of colorectal cancer.