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为探讨丙型肝炎病毒（HCV）感染在肝癌发生中的作用，分析了肝癌患者HCV第二代抗体阳性率，并将HCV－RNA纯化，经随机引物逆转录合成CDNA后进行基因分型，并对核心基因进行表达和测序分析。肝癌患者中HCV抗体阳性率为10．3％，HBV与HCV重叠感染率为4．8％；基因型表现84．2％为Ⅱ型，5．3％为Ⅲ型及10．5％为Ⅱ／Ⅲ混合型。被克隆及序列分析的HCV－N6株与已报道的该病毒Ⅰ型和Ⅱ型的序列比较，其核心基因的核着酸及氨基酸同源性都较低。研究资料提示在南通地区肝癌患者HCV感染率较低，但核心蛋白基因的核着酸置换具有增高趋势。
In order to investigate the role of hepatitis C virus (HCV) infection in the development of hepatocellular carcinoma, the positive rate of HCV second generation antibody in liver cancer patients was analyzed, and HCV-RNA was purified and then genotyped by random primer reverse transcriptase synthesis of CDNA. Core genes were subjected to expression and sequencing analysis. The positive rate of HCV antibody in patients with hepatocellular carcinoma was 10.3%, and the overlapping rate of HBV and HCV was 4.8%; genotype performance was 84.2% in type II, 5.3% in type III and 10.5% in II. /III hybrid type. The cloned and sequenced HCV-N6 strain had lower nucleotide and amino acid homology compared with the reported sequences of type I and type II of the virus. Research data suggests that the prevalence of HCV infection in patients with liver cancer is low in Nantong, but the core protein gene nuclear exchange has an increasing trend.