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目的：了解新生小鼠耳蜗在体外条件下存活状况，为体外急性实验提供组织模型。方法：对出生１～３ｄ小鼠耳蜗以体外悬浮培养方法，并用扫描电镜对其外毛细胞的结构和形态进行观察。结果：采用这种方法培养的外毛细胞，在存活的２０ｈ内，其细胞形态和结构无明显改变。结论：耳蜗悬浮培养对短期离体外毛细胞的研究不失为稳定可靠的方法，而扫描电镜也补充证实为一种有效、简便的观察手段
Objective: To understand the survival of neonatal mouse cochlea in vitro and to provide a tissue model for acute in vitro experiments. Methods: The cochleas of 1 ~ 3 days old mice were cultured in vitro and the structure and morphology of their outer hair cells were observed by scanning electron microscopy. Results: The outer hair cells cultured by this method showed no significant changes in cell morphology and structure within 20 hours of survival. Conclusion: Cochlear suspension culture of short hair cells in vitro after the study is a stable and reliable method, and scanning electron microscopy also confirmed as an effective and easy means of observation