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[目的]对种子带菌作为玉米弯孢菌叶斑病的初侵染源进行研究,为生产上防治该病提供理论依据。[方法]采用玻片萌发法和组织分离法测定种子外部带菌、内部带菌的存活力;采用沙培法在室内检测种子带菌对幼苗的侵染;应用田间培养法测定种子带菌对玉米的侵染。[结果]玉米种子所带弯孢菌孢子和菌丝可以越冬成活,种子内部带菌影响种子萌发,种子外部带菌不影响种子萌发;种子内外带菌均能侵染玉米幼苗的胚根和胚芽。[结论]玉米种子带弯孢菌孢子和菌丝可以越冬并侵染幼苗,使幼苗长势衰弱。
[Objective] The research aimed to study the carrier of seed as the primary infection source of Curvularia lunata leaf spot disease and provide the theoretical basis for the prevention and control of this disease in the production. [Method] The viability of fungi carrying bacteria and internal fungi was determined by slide germination method and tissue separation method. The sand culture method was used to detect the infestation of seedlings by seed carriers. The field culture method was used to determine the infestation of corn by seed carriers . [Result] The spores and mycelium of corn sprouting could survive overwintering. The internal germination of the seeds affected the seed germination. The external germination of the seeds did not affect the seed germination. Both the internal and external seed germs could infect the radicle and germ of the corn seedlings. [Conclusion] The sporophytes and mycelium of corn Curvularia lunata could overwinter and infiltrate the seedlings, making the seedlings weak.