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为了建立一种快速鉴定大麦小孢子来源植株倍性的方法,对大麦品种“花30”不同倍性(种子来源二倍体,小孢子来源单倍体和单倍体加倍系)早期植株的不同叶位、不同叶片部位的气孔保卫细胞长度进行了测定,考察了不同取材部位气孔保卫细胞长度的差异,对种子来源二倍体、小孢子来源单倍体和单倍体加倍材料的气孔保卫细胞长度的分布进行了区分。结果表明,“花30”单倍体材料的气孔保卫细胞长度在不同叶位以及不同叶片部位差异较小,而单倍体加倍材料和种子来源二倍体材料受叶片部位影响较大,单倍体和二倍体材料间气孔保卫细胞的长度差异显著,而单倍体加倍材料和种子来源二倍体材料间气孔保卫细胞长度未观察到明显差异。单倍体和单倍体加倍材料气孔保卫细胞的长度值范围分别为26.9~37.7μm和36.7~62.1μm;利用37μm临界值可对大麦小孢子来源的DH群体中的单倍体进行快速区分。
In order to establish a method for rapid identification of ploidy-derived plants from microspore-derived plantlets of barley, the effects of different ploidy (“diploid”, “microspore derived haploid” and “haploid”) early plants The length of stomatal guard cells at different leaf positions and different leaf parts were measured. The differences of stomatal guard cells length in different accessions were investigated. The stomata of diploid, microspore derived haploid and haploid doubled materials Guard cell length distribution was distinguished. The results showed that the length of stomatal guard cells in the “Huanan 30” haploid material was smaller at different leaf positions and different leaf parts, while the haploid doubled material and seed-derived diploid material were greatly affected by the leaf parts, There was significant difference in the length of stomata guard cells between haploid and diploid materials, but no significant difference was observed in the length of stomata guard cells between haploid and diploid materials. Haploid guard cells of haploid and haploid doubling materials ranged in length from 26.9 to 37.7μm and 36.7 to 62.1μm, respectively. The haplotypes of haploid and haploid DH populations derived from microspore-derived DH populations were quickly distinguished using a 37μm cutoff.