维甲酸诱导HL-60细胞Fas蛋白表达

• 作 者: 曾辉 赵相印 马雅銮 赵如冰 杨敏 张勇 刘复强 高天祥
• 机 构: 首都医科大学附属北京同仁医院,首都医科大学实验中心
• 出 处: 中华血液学杂志
• 发表日期: 1998年05期
• 关键词: 维甲酸 细胞分化 细胞凋亡 自体骨髓移植 流式细胞仪检测 细胞系 基因突变 细胞培养 阳性细胞比例 胞核固缩

目的：探讨全反式维甲酸（ＡＴＲＡ）和９顺式维甲酸（９ｃｉｓＲＡ）对ＨＬ６０细胞Ｆａｓ表达水平及维甲酸对抗Ｆａｓ抗体诱导的细胞凋亡敏感性的影响。方法：用流式细胞仪检测ＨＬ６０细胞膜Ｆａｓ蛋白表达水平；分别采用形态学观察、ＤＮＡ电泳和流式细胞仪检测抗Ｆａｓ抗体诱导的细胞凋亡。结果：ＨＬ６０细胞Ｆａｓ弱表达。经１０－６ｍｏｌ／ＬＡＴＲＡ或９ｃｉｓＲＡ分别预处理４天后，ＨＬ６０细胞Ｆａｓ表达水平及对抗Ｆａｓ抗体诱导凋亡的敏感性均显著提高。９ｃｉｓＲＡ提高ＨＬ６０细胞对抗Ｆａｓ抗体诱导凋亡的敏感性的能力强于ＡＴＲＡ。结论：维甲酸可上调ＨＬ６０细胞表达Ｆａｓ，这可能与维甲酸诱导ＨＬ６０细胞分化相关。进一步深入探讨其分子机制，将为肿瘤治疗，特别是自体骨髓移植中骨髓净化提供新方法

Objective: To investigate the effects of all-trans retinoic acid (ATRA) and 9-cis retinoic acid (9cisRA) on the expression of Fas in HL60 cells and the sensitivity of retinoic acid to antiApoptosis induced by retinoic acid. Methods: Flow cytometry was used to detect the expression of Fas protein in HL-60 cells. Morphological observation, DNA electrophoresis and flow cytometry were used to detect the apoptosis induced by anti-Fas antibody. Results: HL 60 cells Fas expression. After 10-6mol / LATRA or 9cisRA were pretreated for 4 days respectively, the expression level of Fas in HL60 cells and the sensitivity to anti-Fas antibody induced apoptosis were significantly increased. 9  cisRA improve HL  60 cells to Fas antibody sensitivity to induce apoptosis than ATRA. Conclusion: Retinoid can up-regulate the expression of Fas in HL-60 cells, which may be related to retinoic acid-induced HL-60 cell differentiation. To further explore its molecular mechanisms will provide a new method for the treatment of cancer, especially bone marrow purification in autologous bone marrow transplantation