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Schisandra chinensis Turcz.(Baill.) is a plant species with fruits that have been well known in Far Eastern medicine for a long time.It has traditionally been used as a stimulating and fortifying agent in cases of physical exhaustion and to inhibit fatigue.The major bioactive compounds found in S.chinensis are lignans with a dibenzocyclooctadiene skeleton,but little is known about their biosynthesis in plants.S.chinensis is the ideal medicinal plant for studying the biosynthesis of lignans,especially the dibenzocyclooctadiene skeleton.Genomic information for this important herbal plant is unavailable.To better understand the lignan biosynthesis pathway,we generated transcriptome sequences from the fruit during ripening and performed de novo sequence assembly,yielding 136 843 unique transcripts with N50 of 1778 bp.Putative functions could be assigned to 41 824 transcripts (51.57％) based on BLAST searches against annotation databases including GO (Gene ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes).Furthermore,22 candidate cytochrome P450 genes and 15 candidate dirigent proteins genes that were most likely involved in the lignan biosynthesis pathway were discovered based on transcriptome sequencing of S.chinensis.The genomic data obtained from S.chinensis,especially the identification of putative genes involved in the lignan biosynthesis pathway,will facilitate our understanding of lignan biosynthesis at the molecular level.The lignan metabolite profiles were analyzed by metabolomes,the accumulation patterns of 30 metabolites involved in the lignan pathway were studied.Co-expression network of lignan contents and transcriptional changes showed 355 strong correlations (correlation coefficient,R2 ＞ 0.9) between 21 compounds and 153 transcripts.Furthermore,the comprehensive analysis and characterization of the genes involved in lignan pathways and the metabolite profiles of lignans are expected to provide better insight regarding the diversity of the chemical composition,synthetic characteristics,and regulatory mechanisms of this medical herb.