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一、引言ADP-核糖基转移酶(ADPRT)是高等真核细胞的染色质结合酶,它能催化烟酰胺腺嘌呤二核苷酸(N-AD~+,即辅酶I),使之转变为多聚(ADP-核糖)。在此反应中,当ADP-核糖残基通过2″-1′核糖基——核糖配糖键相互连结时,NAD~+的烟酰胺部分被释放出来。(ADP-核糖)多聚物在形成的最初阶段需要使多聚体与染色质蛋白(主要是组蛋白H1和ADPRT 本身)作共价连接。ADP-核糖基化作用可能参与细胞分
I. INTRODUCTION ADP-ribosyltransferase (ADPRT) is a chromatin-binding enzyme in higher eukaryotic cells. It catalyzes the nicotinamide adenine dinucleotide (N-AD~+, coenzyme I) and converts it into Poly(ADP-ribose). In this reaction, when ADP-ribose residues are linked to each other via 2′′-1′-ribonucleoside-ribo-glycosidic bonds, the NAD~+ niacinamide moiety is released. (ADP-ribose) polymer is formed The initial stage of the process requires the covalent attachment of multimers to chromatin proteins (mainly histone H1 and ADPRT itself). ADP-ribosylation may be involved in cell division.