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目的研究健脾方案对肠粘膜上皮甜味觉通路中味觉受体细胞及信号关键分子mRNA表达的影响。方法将功能性腹泻患者分为脾胃虚弱组及脾胃湿热组(各20例),脾胃虚弱组服用健脾I号方,脾胃湿热组服用健脾II号方,每次20m L,3次/d,疗程4周。胃镜下钳取治疗前后十二指肠粘膜组织2块,采用QPCR法检测两组功能性腹泻患者治疗前后十二指肠粘膜味觉通路中味觉受体第一家族(T1R1、T1R2、T1R3)及信号关键分子的表达。结果治疗后除脾胃湿热组肠道味觉受体T1R1外,两组肠道味觉受体(T1R1、T1R2、T1R3)及关键信号分子Gαgust表达均有升高(P<0.01)。治疗后,脾胃虚弱组肠道味觉受体第一家族及信号关键分子表达高于经健脾II号方治疗后的脾胃湿热组(P<0.01)。结论健脾法可有效增加功能性腹泻患者肠道甜味觉受体细胞及关键信号分子mRNA的表达。
Objective To study the effect of spleen-strengthening regimen on the expression of taste receptor cells and signaling molecules in the sweet taste pathway of intestinal epithelial cells. Methods The patients with functional diarrhea were divided into spleen-stomach weakness group and spleen-stomach dampness heat group (20 cases each). The spleen-stomach weakness group was treated with Jianpi I, and the spleen-stomach damp heat group was treated with Jianpi II with 20ml each time. , Treatment for 4 weeks. Gastroscopy underwent two clamps of mucosa before and after treatment, and the first family of taste receptors (T1R1, T1R2, T1R3) and the signal of the taste receptor in duodenal mucosal taste pathway were detected by QPCR before and after treatment in patients with functional diarrhea Expression of key molecules. Results After treatment, the expression of Gut receptors (T1R1, T1R2, T1R3) and Gαgust in intestine of both groups increased (P <0.01) except for the taste receptor T1R1 in the spleen-stomach damp-heat group. After treatment, the expressions of the first family members of gut taste receptor and key signaling molecules in spleen-stomach-weakness group were higher than those in spleen-stomach damp-heat treatment group after treatment with Jianpi II (P <0.01). Conclusion The method of invigorating the spleen can effectively increase the mRNA expression of the sweet taste receptors and key signal molecules in patients with functional diarrhea.