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为了探讨下丘脑内一氧化氮与应激之间的关系，本实验应用NADPH-d组化法和C-fos免疫组化技术相结合的方法，对束缚应激大鼠下丘脑内一氧化氮合酶（NOS）和c-fos蛋白的分布以及两者的共存关系进行了观察。结果表明，大鼠在束缚应激4h后，（1）室旁核大细胞部和视上核可见密集深染的NOS阳性大细胞；（2）室旁核小细胞部、背内侧核、穹窿周核、环状核、腹内侧核腹外侧部、结节内侧核、外侧区、室周区、乳头体前核和内侧核的外侧区等核团出现疏密不等和深浅不一的NOS阳性细胞；（3）C-fos蛋白以室旁核表达最为强烈，视前区、背内侧核、弓状核和外侧区亦有较强的表达；（4）在外侧区、室旁核小细胞部及其附近的室周区、背内侧核及乳头体前核腹侧部约有10％～15％的中、小型NOS阳性细胞同时表达C-fos蛋白，室旁核大细胞部则仅有较少的NOS阳性大细胞表达C－fos蛋白，在结节区、结节内侧核和视上核视交叉后部则偶见双标细胞。结果提示上述下丘脑核团内的部分一氧化氮能神经元与束缚应激反应有关。
In order to investigate the relationship between nitric oxide and stress in the hypothalamus, we used the combination of NADPH-d and C-fos immunohistochemistry to study the hypothalamic nitric oxide The distribution of coenzyme (NOS) and c-fos protein and their coexistence were observed. The results showed that after 4h restraint stress in rats, (1) densely stained NOS positive macrophages were observed in the para-nucleus and supraoptic nucleus of the paraventricular nucleus; (2) the paraventricular nucleus, dorsal medulla, and fornix The nuclei such as peripheral nucleus, annular nucleus, ventral portion of ventral medial nucleus, medial nodules, lateral region, periventricular region, lateral nucleus of prepumenal papillae and medial nucleus showed sparse and dense NOS Positive cells; (3) C-fos protein was most strongly expressed in the paraventricular nucleus, and was also expressed in the anterior optic nerve, dorsal medial nucleus, arcuate nucleus and lateral area; (4) In the lateral area, Around the cells and around the ventricular zone, the dorsal medulla nucleus and the ventral part of the nipple anterior nucleus about 10% to 15% of small and medium NOS positive cells express C-fos protein, while the paraventricular nucleus large cell only Fewer NOS positive macrophages express C-fos protein, occasionally double-labeled cells in the nodal area, the medial nodules and the supraoptic nucleus. The results suggest that some nitric oxide neurons in the hypothalamic nucleus are associated with restraint stress.