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目的 探讨三七总皂苷(PNS)对脂多糖(LPS)诱导的小鼠抑郁样行为及脑内小胶质细胞活化的影响.方法 24只雄性昆明小鼠随机分为溶媒对照(NS)组、LPS组、三七总皂苷预处理(PNS+ LPS)组,采用新奇环境抑制摄食实验、糖水偏好实验、悬尾实验、强迫游泳实验观察小鼠的抑郁样行为;采用免疫组织化学法检测海马、杏仁核小胶质细胞标记物离子钙接头蛋白分子1(IBA-1)的表达变化.结果 LPS导致小鼠出现抑郁样行为,包括新奇环境摄食潜伏期延长(P＜0.05),糖水偏好百分比下降(P＜0.05),悬尾不动时间延长(P＜0.05),强迫游泳不动时间增加(P＜0.01),PNS干预可逆转上述抑郁行为表现(P＜0.05);LPS导致小鼠海马和杏仁核IBA-1阳性细胞数目显著增多(P ＜0.0001),PNS预处理能明显减少IBA-1的阳性细胞数(P＜0.05).结论 三七总皂苷能有效缓解LPS诱导的小鼠抑郁行为,其机制可能与抑制脑内小胶质细胞活化有关.“,”Objective To investigate the effect of Panax notoginseng total saponins (PNS) on lipopolysacchafide (LPS)-induced depression-like behavior and expression of microglia in mice.Methods Totally 24 male Kunming mice were randomly divided into NS group,LPS group and PNS + LPS group.Depression-like behavior was investigated by novelty-suppressed feeding test,sucrose preference test,tail suspension test and forced swimming test.Expression of microglia marker ionized calcium binding adaptor molecule-1 (IBA-1) in the hippocampus and amygdala was determined by Immunohistochemistry assess.Results LPS led to prolonged latency in the novelty-suppressed feeding test (P ＜ 0.05),reduced sucrose preference (P ＜ 0.05),and increased immobility time in the tail suspension test (P ＜ 0.05) and forced swimming test (P ＜ 0.01),while PNS treatment could effectively reverse the depression-like behavior induced by LPS injection (P ＜ 0.05).LPS injection led to significantly increased number of IBA1 positive cells in the hippocampus and amygdala (P ＜ 0.0001),which was attenuated by PNS treatment (P ＜ 0.05).Conclusion Our findings indicate that PNS can alleviate LPS-induced depression-like behavior in mice,which may be attributed to the inhibiting of microglia activation in the hippocampus and amygdala.