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AIM: To investigate the effects of inositol hexaphosphate (IP6) on proliferation of HT-29 human colon carcinoma cell line. METHODS: Cells were exposed to various concentrations (0, 1.8, 3.3, 5.0, 8.0, 13.0 mmol/L) of IP6 for a certain period of time. Its effect on growth of HT-29 cells was measured by MTT assay. The expressions of cell cycle regulators treated with IP6 for 2 d were detected by immunocytochemistry. RESULTS: IPe inhibited the HT-29 cell growth in a dose- and time-dependent manner. Analysis of cell cycle regulator expression revealed that IPe reduced the abnormal expression of P53 and PCNA and induced the expression of P21. CONCLUSION: IPe has potent inhibitory effect on proliferation of HT-29 cells by modulating the expression of special cell cycle regulators.
METHODS: Cells were exposed to various concentrations (0, 1.8, 3.3, 5.0, 8.0, 13.0 mmol / L) of IP6 Its effect on growth of HT-29 cells was measured by MTT assay. The expressions of cell cycle regulators treated with IP6 for 2 d were detected by immunocytochemistry. RESULTS: IPe inhibited the HT-29 cell growth in a dose- and time-dependent manner. Analysis of cell cycle regulator expression revealed that IPe reduced the abnormal expression of P53 and PCNA and induced the expression of P21. CONCLUSION: IPe has potent inhibitory effect on proliferation of HT-29 cells by modulating the expression of special cell cycle regulators.