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目的:探讨抗核小体抗体(ANuA)的血清水平检测在狼疮肾炎(LN)患者诊断及肾脏病理改变中的临床意义。方法:选择本院2010年5月~2012年6月确诊收治的系统性红斑狼疮(SLE)患者125例,其中84例合并狼疮肾炎(A组),41例未合并狼疮肾炎(B组),另选择同期收治其他类型的免疫性疾病患者55例(C组)作对照,采用酶联免疫吸附试验(ELISA法)对抗核小体抗体水平进行检测。结果:A和B组阳性率分别为94.2%和90.2%,差异不显著(P>0.05),但均高于C组54.5%(P<0.01)。且3组间ANuA水平差异显著,其中A组最高,C组最低(P<0.05)。各类型肾脏改变中ANuA水平差异显著(P<0.05),其中以Ⅳ型患者血清中ANuA含量最高(P<0.05)。结论:血清抗核小体抗体与狼疮肾炎的发生密切相关,可反映肾脏病变程度,对病情监测有重要意义。
Objective: To investigate the clinical significance of detecting serum levels of anti-nucleosome antibody (ANuA) in diagnosis of patients with lupus nephritis (LN) and pathological changes of kidney. Methods: A total of 125 patients with systemic lupus erythematosus (SLE) admitted to our hospital from May 2010 to June 2012 were enrolled. Among them, 84 patients were complicated with lupus nephritis (group A), 41 patients without lupus nephritis (group B) In addition, 55 patients with other types of autoimmune diseases were selected as controls in the same period (C group), and the levels of anti-nucleosome antibodies were detected by enzyme linked immunosorbent assay (ELISA). Results: The positive rates of A and B were 94.2% and 90.2% respectively, with no significant difference (P> 0.05), but both of them were higher than those of C (54.5%, P <0.01). There was significant difference in ANuA level between the three groups, with the highest in group A and the lowest in group C (P <0.05). The level of ANuA in all types of kidney changes was significantly different (P <0.05), among which the level of ANuA in type Ⅳ patients was the highest (P <0.05). Conclusion: Serum anti-nucleosomal antibodies are closely related to the occurrence of lupus nephritis, which can reflect the degree of renal disease and have important significance for disease surveillance.