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目的通过对原发癌及骨转移瘤患者外周血淋巴细胞姐妹染色单体交换(sister chromatid exchanges,SCE)率和微核(micronuclei,MN)率的研究,来探讨细胞癌变中DNA的变化。方法检测正常人、原发癌和骨转移癌的外周血淋巴细胞姐妹染色单体交换率和微核率,并比较结果。结果有原发肿瘤的患者姐妹染色单体交换率和微核率增高,而骨转移瘤患者的姐妹染色单体交换率和微核率与无转移瘤患者比较,两者存在显著差异。结论骨转移瘤患者的姐妹染色单体交换率和微核水平损伤严重,姐妹染色单体交换率和微核率能敏感反映DNA的损伤情况,故此姐妹染色单体交换率和微核率检测被用作快速检测环境中诱变、致癌因素的灵敏方法之一。
Objective To investigate the changes of DNA in cell carcinomas by studying sister chromatid exchanges (SCE) rates and micronuclei (MN) rates of peripheral blood lymphocytes in patients with primary carcinoma and bone metastases. Methods The sister chromatid exchange rate and micronucleus rate of peripheral blood lymphocytes of normal, primary and metastatic bone cancer were detected and compared. Results Sister chromatid exchange rate and micronucleus rate were increased in patients with primary tumor, while sister chromatid exchange rate and micronucleus rate in patients with bone metastases were significantly different from those without metastases. Conclusion The sister chromatid exchange rate and micronucleus level of patients with bone metastases are severely damaged. The sister chromatid exchange rate and micronucleus rate can sensitively reflect the DNA damage. Therefore, the detection rate of sister chromatid exchange rate and micronucleus rate Used as a sensitive method for rapid detection of mutagenic, carcinogenic factors in the environment.