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合成了二茂铁接枝聚乙烯亚胺(PEI-Fc),利用二茂铁与β-环糊精的主客体嵌套作用制备了环糊精修饰聚乙烯亚胺,核磁测定结果显示,每条PEI-Fc链上通过主客体作用嵌套的CD平均为26个.这种基于弱相互作用力的β-环糊精修饰聚乙烯亚胺能有效诱导DNA分子的缔合,在N/P值达到3以上时,可形成表面为正电荷、粒径为150～250 nm的球形粒子.在含10%胎牛血清的DMEM体外细胞培养基中,由于培养基中的蛋白质能够在粒子表面发生静电吸附,PEI-Fc/CD/DNA基因微载体显示出良好的稳定性.HEK293细胞培养结果显示,以表达绿色荧光蛋白的质粒pEGFP为模型,以N/P值为10的PEI/DNA组装体作为对照,N/P值为3、5和10的PEI-Fc/CD/DNA组装体的转染效率均达到对照组的2～3倍,这种基于主客体组装构建的环糊精修饰基因微载体显著提高了基因转染效率。
Ferrocene-grafted polyethyleneimine (PEI-Fc) was synthesized. Cyclodextrin-modified polyethyleneimine was prepared by the host-guest nested reaction of ferrocene with β-cyclodextrin. The results of nuclear magnetic resonance The average number of CDs nested on the PEI-Fc chain through host-guest interactions was 26. This weakly interacting β-cyclodextrin-modified polyethyleneimine effectively induced the association of DNA molecules, with N / P When the value is more than 3, spherical particles with a positive charge of 150-250 nm can be formed in DMEM in vitro cell culture medium containing 10% fetal bovine serum, since the protein in the medium can occur on the particle surface PEI-Fc / CD / DNA microcarriers showed good stability by electrostatic adsorption.The results of HEK293 cell culture showed that the green fluorescent protein plasmid pEGFP was used as the model and PEI / DNA assembly with N / P value of 10 As a control, PEI-Fc / CD / DNA assemblies with N / P values of 3, 5, and 10 transfection efficiency of 2 to 3 times that of the control group. This cyclodextrin-modified gene Microcarriers significantly improve gene transfection efficiency.