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目的:研究不同剂量天钩降压胶囊对大鼠体内丹皮酚药动学的影响。方法:大鼠分别灌胃给予低、中、高剂量天钩降压胶囊(相当于丹皮酚6.884,13.77,27.53 mg.kg-1),于给药后不同时间采集大鼠血浆。血浆样品用甲醇沉淀蛋白,HPLC测定丹皮酚含量,各给药组平均血药浓度-时间数据采用DAS2.0软件分析。组间药动学参数用SPSS 16.0软件进行统计分析。结果:低剂量组大鼠丹皮酚血药浓度在消除相超出最低检测限,未能测出药时曲线;中、高剂量组丹皮酚均在5 min达最大吸收;t1/2ka,Cmax,AUC0-∞,AUC0-360,CLz/F随给药剂量增加而显著性增加;t1/2z,MRT0-360,Vz/F随给药剂量增加而显著性减小。结论:大鼠灌胃不同剂量天钩降压胶囊后丹皮酚的药动学参数存在一定差异。
Objective: To study the effect of different doses of Tian hookjiangya capsule on the pharmacokinetics of paeonol in rats. Methods: Rats were administered intragastrically with low, medium and high doses of Tian hook hypotensive capsules (equivalent to paeonol 6.884,13.77,27.53 mg.kg-1), at different times after administration of rat plasma was collected. Plasma samples were precipitated with methanol, and the content of paeonol was determined by HPLC. The average plasma concentration-time data of each administration group was analyzed by DAS2.0 software. Pharmacokinetic parameters between groups were analyzed by SPSS 16.0 software. Results: The plasma concentration of paeonol in the low-dose group exceeded the lowest detection limit in the elimination phase, and the drug-free curve was not obtained. Paeonol in the middle-dose and high-dose groups reached maximum absorption at 5 min; t1 / 2ka, Cmax , AUC0-∞, AUC0-360, CLz / F increased significantly with the increase of dose; t1 / 2z, MRT0-360, Vz / F decreased significantly with the dose increased. Conclusion: The pharmacokinetic parameters of paeonol in rats after intragastric administration of Tian hookjiangya capsule with different doses have some differences.