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[目的]探讨缺氧诱导因子-1α(HIF-1α)在宫颈癌和宫颈上皮内瘤变中的表达及其生物学意义。[方法]选取1996年至2004年诊断为宫颈癌的存档组织蜡块78例,CIN40例,正常宫颈组织12例。41例新鲜宫颈组织和液基细胞标本取自2005年至2006年手术切除标本。利用免疫组织化学、Western blot和RT-PCR等方法检测宫颈癌、宫颈上皮内瘤变、癌旁组织、正常组织和液基细胞中的HIF-1α表达。[结果]宫颈癌、CINⅡ￣Ⅲ、CINⅠ和正常组织中HIF-1α的阳性表达率分别为58.9%(46/78)、48.0%(12/25)、6.67%(1/15)、0(0/12);宫颈癌组织中,HIF-1α表达与肿块大小、FIGO分期、组织学分级和淋巴结转移密切相关(P<0.05),与患者年龄无关;宫颈癌组织和CINⅡ￣Ⅲ中的mRNA和蛋白水平均高于癌旁和正常组织及CINⅠ(P<0.05);液基细胞中宫颈癌和HSIL的mRNA和蛋白水平都高于LSIL及正常细胞(P<0.05)。[结论]HIF-1α可能是宫颈癌形成过程中的早期分子事件,其表达增强与宫颈癌的浸润性发展密切相关,有可能成为新的早期筛查和判断宫颈癌生物学行为的诊断指标。
[Objective] To investigate the expression of hypoxia inducible factor-1α (HIF-1α) in cervical carcinoma and cervical intraepithelial neoplasia and its biological significance. [Methods] Seventy-eight archived wax blocks, 40 cases of CIN and 12 cases of normal cervical tissue were selected from 1996 to 2004 for diagnosis of cervical cancer. 41 cases of fresh cervical tissue and liquid-based cell samples taken from 2005 to 2006 surgical specimens. The expression of HIF-1α in cervical cancer, cervical intraepithelial neoplasia, paracancerous tissues, normal tissues and liquid-based cells was detected by immunohistochemistry, Western blot and RT-PCR. [Results] The positive rates of HIF-1α in cervical cancer, CINⅡ-Ⅲ, CINⅠ and normal tissues were 58.9% (46/78), 48.0% (12/25), 6.67% (1/15) 0/12). The expression of HIF-1α in cervical cancer was closely related to tumor size, FIGO stage, histological grade and lymph node metastasis (P <0.05) (P <0.05). The mRNA and protein levels of cervical cancer and HSIL in liquid-based cells were higher than those in LSIL and normal cells (P <0.05). [Conclusion] HIF-1α may be an early molecular event in the process of cervical cancer formation. The increased expression of HIF-1α is closely related to the invasiveness and development of cervical cancer, which may be a new diagnostic indicator for early screening and judging the biological behavior of cervical cancer.